邻苯二甲酸二(2-乙基己)酯对小鼠睾丸损伤及p-CREB蛋白表达的影响

doi:10.3969/j.issn.1004-616x.2019.04.010

Abstract

Abstract: OBJECTIVE:To investigate the effects of di-(2-ethylhexyl)phthalate (DEHP) on spermatogenic function and expression of p-CREB in testes of mice. METHODS:40 newly weaned ICR male mice were treated with DEHP by gavage in doses of 0,250,500,1 000 mg/kg,6 times a week for 8 consecutive weeks. The weights of testes,epididymis and sperm counts were determined. Histomorphology and expressions of p-CREB of testicle tissues were also analyzed. RESULTS:Results:Compared with the control group,the epididymis coefficient and sperm counting of DEHP treated groups were all reduced significantly (P < 0.05). The testicular coefficients were also decreased in the 1000 mg/kg group (P < 0.05). Pathological alterations of testes were also observed. Reduced seminiferous epithelium,loose and anomalistic organization were observed in all treatment groups. Sperm counts were reduced in the lumina of seminiferous tubules. p-CREB protein expressions were down-regulated in the 500 and 1 000 mg/kg exposure groups (P < 0.05). CONCLUSION:Our data show that exposure in mice to DEHP damaged epithelial cells of seminiferous tubule,decreased the number of spermatozoa and the expression of p-CREB protein.

Key words: di-(2-ethylhexyl)phthalate, testicle, spermatogenic function, p-CREB

CLC Number:

R394.6

BO Cunxiang, ZHANG Yu, BAI Jin, HAN Ru, CHEN Shangya, SAI Linlin. Effects of di-(2-ethylhexyl) phthalate on testicular injury and on expression of p-CREB in testes of mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 319-322,330.

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Effects of di-(2-ethylhexyl) phthalate on testicular injury and on expression of p-CREB in testes of mice

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