肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

doi:10.3969/j.issn.1004-616x.2019.04.005

Abstract

Abstract: OBJECTIVE:To investigate the effect of phenylethanol glycoside liposomes from Cistanche (CPhG) on proliferation,apoptosis and cell cycle of HSC-T6. METHODS:HSC cultures were divided into untreated control and treated with low,medium and high concentrations of CPhG liposomes (7.36,14.72,29.45 μg/mL,respectively). Growth inhibition was detected by using the MTT assay,apoptosis was evaluated by Annexin V-FITC/PI staining and cell cycle changes were detected by flow cytometry. Protein expressions of cleaved caspase-3,p27 were detected using Western blotting. RESULTS:Compared with the control group,all treatment doses significantly inhibited cell proliferation and promoted apoptosis (P < 0.05). In addition,cells were arrested in the G0/G1 phase and showed significant up-regulated expression of caspase-3 and p27 proteins (P < 0.05). CONCLUSION:CPhG liposomes inhibited HSC cell proliferation,induced apoptosis and caused cell cycle arrest by up-regulating the protein expression of caspase-3 and p27.

Key words: Cistanche phenylethanoid glycosides, liposomes, HSC-T6, proliferation, apoptosis, cell cycle

CLC Number:

R322.47

ZHANG Shilei, YOU Shuping, MA Xiaoting, MA Long, ZHAO Jun, LIU tao. Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 289-294.

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Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro

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