白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响

doi:10.3969/j.issn.1004-616x.2019.04.004

Abstract

Abstract: OBJECTIVE:To investigate the effects of resveratrol (RSV) on the genome instability (GIN) and proliferation of human colonic epithelial cell line NCM460 and colon cancer cell line SW620. METHODS:Effects of different concentrations of RSV (3.125,6.25,12.5,25,50,100 μmol/L) on cell proliferation were detected using the trypan blue exclusion method. CBMN-Cyt and colony formation experiments were performed to examine the effects of different concentrations of RSV (25,100 μmol/L) on GIN and clonal formation ability. RESULTS:Compared with the control group,RSV of 6.25-100 μmol/L significantly inhibited the proliferation of SW620 cells (P < 0.05). RSV of 100 μmol/L significantly increased the apoptosis rates,increased the GIN of SW620 cells and decreased the ability of clonal formation (P < 0.05 or 0.01). On the other hand,the same concentration of RSV decreased the GIN of NCM460 cells,enhanced cell division and cell clonality (all P < 0.05). CONCLUSION:The data show that RSV was able to maintain the genomic stability of the normal NCM460 epithelial cells but significantly increased the incidence of GIN in the SW620 colon cancer cells which could be the molecular basis for its excellent anticancer effect and normal cell protection in colon cancer therapy.

Key words: resveratrol, cell proliferation, genomic instability, apoptosis, colony formation assay

CLC Number:

R73-3

CAO Yu, ZHANG Qiaoye, HUA Rui, MA Xiaoling, WANG Xu, NI Juan. Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 283-288.

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Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620

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