纳米二氧化钛对IEC-6细胞炎症因子表达及JAK2/STAT3蛋白磷酸化的影响

doi:10.3969/j.issn.1004-616x.2019.02.006

Abstract

Abstract: OBJECTIVE:To investigate the effects of nano-TiO₂ on expression of inflammatory cytokines and phosphorylation of JAK2/STAT3 in IEC-6 cells.METHODS:IEC-6 cells in cultures were treated with different doses (0.0,5.0,10.0,15.0,20.0 μg/mL) of nano-TiO₂ for 24 h.MTT assay was used to detect cell viability.The levels of inflammatory cytokines (IL-1β,IL-6 and TNF-α) were determined by ELISA assay.Protein expression and phosphorylated levels of JAK2 and STAT3 were measured by Western blot and the gray values of protein bands were assessed by Image J software.RESULTS:Compared with the control group,viability of IEC-6 cells was barely affected by nano-TiO₂.From the treatments with all doses of nano-TiO₂,IL-6 and TNF-α were highly secreted (P < 0.05),and significant secretion of IL-1β with the exception from the lowest dose of TiO₂,(P < 0.05).Western bolt results indicate the levels of p-JAK2 and p-STAT3 were increased after exposure of nano-TiO₂ and had a positive relationship with expressions of secreted inflammatory factors (r_JAK2,IL-1β=0.561,P < 0.05;r_JAK2,IL-6=0.711,P < 0.01;r_JAK2,TNF-α=0.675,P < 0.01;r_STAT3,IL-1β=0.782,P < 0.01;r_STAT3,IL-6=0.532,P < 0.05;r_STAT3,TNF-α=0.668,P < 0.01).The gray values of phosphorylated JAK2 and STAT3 increased when IEC-6 cells were exposed to 15.0 and 20.0 μg/mL nano-TiO₂(P < 0.05).CONCLUSION:Nano-TiO₂ exposure caused significant perturbation in expression of inflammatory cytokines and phosphorylation of JAK2 and STAT3 in IEC-6 cells.

Key words: nano-TiO₂, inflammation, JAK2/STAT3, IEC-6 cell

CLC Number:

LIU Jing, ZHANG Xiaoqiang, WANG Xu, MIAO Xinyu, LU Xiaoxing. Titanium dioxide nanoparticles on expression of inflammatory factors and phosphorylation of JAK2/STAT3 in IEC-6 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 127-132.

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Titanium dioxide nanoparticles on expression of inflammatory factors and phosphorylation of JAK2/STAT3 in IEC-6 cells

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