小鼠肝再生过程中MnSOD作用的初步研究

doi:10.3969/j.issn.1004-616x.2019.01.011

Abstract

Abstract: OBJECTIVE:The aim of this study was to investigate changes of MnSOD expression and its activity in liver regeneration after partial hepatectomy. METHODS:Using the classic mouse partial hepatectomy model,38 male BALB/c mice were randomly divided into 30% partial hepatectomy group (30%PH) and 70% partial hepatectomy group (70%PH). There were 18 mice in each experimental group,and 2 as sham operation control group. 3 mice was randomly selected from the two hepatectomy groups at 6 h,1 d,2 d,3 d,5 d and 7 d after the operation,and the control group was executed immediately after the sham operation. Liver tissues in the PH groups were harvested at 6 h,1 d,2 d,3 d,5 d and 7 d respectively. Frozen sections from collected tissues were used to detect the level of reactive oxygen species by the DHE dyeing method and using laser confocal microscopy. MnSOD mRNA was measured by Real Time Q-PCR. Expression of MnSOD protein was determined by Western blot,and activity of MnSOD was assessed using the MnSOD kit. RESULTS:Compared with the control group,the ROS levels in liver tissues from PH mice increased at 6 h and 1 d after operation,expression level of MnSOD mRNA increased (P < 0.05),but protein content of MnSOD did not change significantly in the 30%PH group (P > 0.05). Activities of MnSOD was higher on 1 d and 2 d,lower on 3 d and 5 d,and then recovered on 7 d after 30%PH. In the 70%PH group,ROS level in liver tissues increased from 1 d to 5 d after operation; MnSOD mRNA levels decreased first and then gradually recovered; protein content of MnSOD decreased; and activities of MnSOD increased at 6 h and 1 d,and then decreased during 2 d to 7 d (all P < 0.05). CONCLUSION:Liver regeneration started rapidly after hepatectomy in mice,especially after 70%PH. The hepatocytes rapidly proliferated,and then gradually returned to a resting state after a period of time. The observed changes might be related to the down-regulation of MnSOD content and activity,which led to the increase of ROS.

Key words: MnSOD, partial hepatectomy, liver regeneration, reactive oxygen species, cell proliferation

CLC Number:

R364.3

LI Ge, WANG Yaofei, GAN Na, HE Qi, WANG Guan, HAN Zhanfeng, HAI Chunxu, LIN Zhaoxing. Preliminary study on the role of MnSOD in liver regeneration[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 58-63,78.

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Preliminary study on the role of MnSOD in liver regeneration

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