HepG2细胞外泌体冲击树突状细胞介导的抗肿瘤作用

doi:10.3969/j.issn.1004-616x.2019.01.005

Abstract

Abstract: OBJECTIVE:To investigate the liver cancer cell HepG2-dervied immunogenic substances as tumor antigens for dendritic cell-(DC) based immunotherapy of liver cancer. METHODS:Changes in morphology and protein expression of HepG2 cells were determined by transmission electron microscopy and western blot,respectively; and in surface molecules of DCs by flow cytometry using exosomes with a final concentration of 8 μg/mL. In addition,exosome-pulsed DCs were co-cultured with T lymphocytes for 5 days. Proliferation of lymphocytes was detected by CSFE staining,and their killing of tumors was detected by Annexin-V/PI double staining. RESULTS:The hepatoma cell HepG2 exosomes expressed the marker proteins CD63 and CD81,carried a large number of tumor antigens AFP and heat shock proteins HSP70 and HSP90,but did not express the cellular protein calnexin. Compared with the DC-MOCK group,exosome-pulsed DCs up-regulated the expression of surface molecules such as CD83,CD80,and CD86; promoted T lymphocyte proliferation (P < 0.01),and increased T cell-mediated tumor-specific and non-specific killing effects (P < 0.01). CONCLUSION:Hepatoma cell HepG2 exosomes carried a large number of tumor antigens and stimulated the maturation of DCs,which may serve as a potential antigenic spectrum of DCs-based immunotherapy for liver and other cancers.

Key words: exosomes, hepatocellular carcinoma, dendritic cells, HepG2 cells

CLC Number:

R730.5

ZHANG Ju, YE Shulai, ZHANG Changlong, ZHOU Qian. Stimulation of dendritic cell-mediated antitumor tumor effect from HepG2 cell exosomes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 29-34.

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Stimulation of dendritic cell-mediated antitumor tumor effect from HepG2 cell exosomes

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