PD98059抑制MAPK/ERK信号通路对胃癌细胞生物学功能的影响

doi:10.3969/j.issn.1004-616x.2019.01.003

Abstract

Abstract: OBJECTIVE:To investigate expression of key signaling molecules MEK and ERK in the MAPK/ERK signaling pathway and effect of PD98059 on MAPK/ERK pathway's biological function in the gastric cancer SGC-7901 cells. METHODS:Gastric cancer cell line SGC-7901 was cultured in vitro. Cell proliferation rates were detected by the CCK-8 method after treatment with different concentrations of PD98059 at 0,25,50,100,200,300 and 400 μmol/L for 24 h. Expression of MEK and ERK mRNA was detected by real-time quantitative PCR (qPCR) after treatment with 0,25,50 and 100 μmol/L PD98059 for 24 h. Expression of MEK and ERK proteins was detected by Western blot. Cell cycle and apoptosis were detected by flow cytometry. The normal gastric mucosal epithelial GES-1 cells were used as controls. RESULTS:Compared with the normal cells,expression of MEK and ERK mRNA and p-MEK and p-ERK proteins in the gastric cancer cells was significantly increased (P < 0.05). After treatment with PD98059,the cell proliferation rates were decreased with increasing concentration and in a dose-dependent manner. When the concentration of PD98059 was between 200 and 400 μmol/L,the inhibition became stabilized. Expression of the MEK and ERK mRNA was lower than that of the control group after treatment with 0-100 μmol/L PD98059 (P < 0.05). With increasing concentrations of PD98059,expression of ERK mRNA was gradually reduced (P < 0.05). Western blot analyses show that expression of p-MEK1/2 and p-ERK1/2 protein decreased after treatment with 50 and 100 μmol/L PD98059 (P < 0.05). Moreover,PD98059 caused G0/G1 phase arrest and induced apoptosis. CONCLUSION:MAPK/ERK signaling pathway was active in the gastric cancer cells. PD98059 inhibited activities in the MAPK/ERK signaling pathway and affected the biological function of the gastric cancer cells.

Key words: gastric cancer, MAPK/ERK signaling pathway, PD98059, cell proliferation, apoptosis

CLC Number:

R730.2

LIU Mengqi, ZHANG Wenwen, CHEN Xiaowei, SHEN Xiaobing. Inhibitory effects of PD98059 on the MAPK/ERK signaling pathway in gastric cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 15-21.

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Inhibitory effects of PD98059 on the MAPK/ERK signaling pathway in gastric cancer cells

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