阿尔泰金莲花浸膏粉对PM2.5致大鼠急性肺损伤的保护作用

doi:10.3969/j.issn.1004-616x.2019.001.004

Abstract

Abstract: OBJECTIVE:To study protective effect of Trollius altaicus extract powder (TAEP) on PM2.5-induced acute lung injury (ALI) in rats. METHODS:Sixty SD rats were randomly divided into normal control (normal saline),model (normal saline),TAEP 125 mg/kg,250 mg/kg,500 mg/kg,and dexamethasone positive control (2 mg/kg) groups. Each dose group was orally administered with the test substance for 30 days. Except the normal control group,the other groups were instilled with PM2.5 suspension on the 30th day,causing acute lung injury in rats. Blood samples were collected from the abdominal aorta. Leukocyte count was performed and serum lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione(GSH) and malondialdehyde(MDA) were analyzed. Nitric oxide(NO),interleukin-6(IL-6),interleukin-1β(IL-1β),TNF-alpha(TNF-α) were measured. The right upper lobe was taken for histopathological examination,the lower lobe was weighed to calculate the wet/dry weight ratio (W/D),the left lung was taken for alveolar lavage,and the alveolar lavage fluid (BALF) was collected to detect the contents of IL-6,IL-1beta and TNF-alpha. RESULTS:Compared with the normal control group,the white blood cell count,IL-6,IL-1β and TNF-α levels in serum and BALF were increased (P < 0.01),and serum LDH,MDA and NO levels were increased (P < 0.01),the W/D of lung tissue increased (P < 0.01),and the content of SOD and GSH decreased (P < 0.01). Compared with the model group,the white blood cell count of each dose of TAEP,IL-6 and IL in serum and BALF -1β,TNF-α decreased (P < 0.05 or P < 0.01),serum LDH,MDA,NO content decreased (P < 0.05 or P < 0.01),and lung tissue W/D decreased (P average < 0.05 or P < 0.01),SOD and GSH levels increased (P < 0.05 or P < 0.01); TAEP 500 mg/kg group decreased white blood cell count,serum,BALF IL-1β,IL-6,TNF -α content decreased,serum LDH,MDA,NO content decreased,SOD,GSH content increased,lung W/D decreased compared with dexamethasone group (P < 0.05). CONCLUSION:The results indicate that the Trollius altaicus extract powder has a protective effect on PM2.5-induced lung injury in rats.

Key words: Trollius altaicus, extract powder, PM2.5, acute lung injury, protection

CLC Number:

R285.5

CHEN Wenlong, YOU Shuping, ZHAO Jun, ZHANG Jie, LIU Tao. Effects of Trollius altaicus extract powder on PM2.5-induced lung injury in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 22-28.

References

[1] 杨洪斌,邹旭东,汪宏宇,等. 大气环境中PM2.5的研究进展与展望[J]. 气象与环境学报,2012,28(3):77-82.
[2] 石华东. 室内空气PM2.5污染的国内研究现状及综合防控措施[J]. 环境科学与管理,2012,37(6):111-114.
[3] 丛鲁红,李晨,陈德生,等. 可吸入颗粒物PM2.5对大鼠急性肺损伤及肺纤维化的影响[J]. 中国医师杂志,2016,18(6):857-860,864.
[4] ELICKER B M,JONES K T,NAEGER D M,et al. Imaging of acute lung injury[J]. Radiol Clin N Am,2016,54(6):1119-1132.
[5] 宋冬梅,孙启时. 阿尔泰金莲花化学成分的研究(Ⅰ)[J]. 中国药物化学杂志,2004,14(4):233-235.
[6] 丁万隆,陈震,杨春清. 新疆金莲花属药用植物资源与利用[C]//第二届中国甘草学术研讨会暨第二届新疆植物资源开发、利用与保护学术研讨会论文摘要集. 新疆石河子, 2004:93.
[7] 曹欣欣,王书华. 金莲花药理活性及临床应用研究进展[J]. 神经药理学报,2014,4(4):59-64.
[8] 刘平,陈光晖,邓淑华,等. 金莲花总黄酮抗菌作用的实验研究[J]. 中国实验方剂学杂志,2013,(6):207-210.
[9] 霍潘峰,滕亮,李倩,等. 高速逆流色谱分离纯化阿尔泰金莲花中的活性成分[J]. 新疆医科大学学报,2017,(9):77-80.
[10] 霍潘峰,滕亮,胡旭,等. 阿尔泰金莲花总黄酮及其荭草素2'-O-β-L-半乳糖苷的提取工艺[J]. 医药导报,2018,37(7):869-873.
[11] SZARKA R J,WANG N,GORDON L,et al. A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation[J]. J Immunol Methods,1997,202(1):49-57.
[12] MA J H,SONG S H,GUO M,et al. Long-term exposure to PM2.5 lowers influenza virus resistance via down-regulating pulmonary macrophage Kdm6a and mediates histones modification in IL-6 and IFN-β promoter regions[J]. Biochem Biophys Res Commun,2017,493(2):1122.
[13] 田燕歌,李亚,李建生,等. PM2.5影响呼吸系统疾病的机制探讨[J]. 中医学报,2014,29(12):1721-1723.
[14] 青卉,王向东,张罗. PM2.5与呼吸系统疾病的关系[J]. 国际耳鼻咽喉头颈外科杂志,2016,40(5):311-314.
[15] 中国环境保护总局. HJ/T 374-2007总悬浮颗粒物采样器技术要求及检测方法[S]. 北京:中国标准出版社,2007.
[16] 袁利. 急性肺损伤发病机制研究进展[J]. 国际儿科学杂志,2017,44(6):382-385.
[17] 刘舒洁. 急性肺损伤药物治疗研究进展[J]. 中国处方药,2018,16(6):20-21.
[18] 何梦静,周威. 糖皮质激素的临床应用[J]. 中国临床研究,2018,31(5):679-681.
[19] 赵爱斌,汪岩. 大鼠灌胃注射用地塞米松磷酸钠肠道吸收情况的研究[J]. 山西职工医学院学报,2018,28(1):36-37.
[20] RIVA D R,MAGALHÃES C B,LOPES A A,et al. Low dose of fine particulate matter PM2.5 can induce acute oxidative stress,inflammation and pulmonary impairment in healthy mice[J]. Inhal Toxicol,2011,23(5):257-267.
[21] 闫庆倩,赵学彬,杨莉,等. 不同地区大气PM2.5致大鼠肺损伤的比较实验研究[J]. 环境与健康杂志,2012,29(1):7-11.
[22] BOTELHO F M,JAVIER R M,DOMINIK F,et al. Pulmonary expression of oncostatin M (OSM) promotes inducible BALT formation independently of IL-6,despite a role for IL-6 in OSM-driven pulmonary inflammation[J]. J Immunol Res,2013,191(3):1453-1464.
[23] 廖美娟,赵伟成,梁幸甜,等. 姜黄素对肠缺血再灌注继发性肺损伤大鼠TNF-α与IL-6表达的影响[J]. 临床与病理杂志,2018,38(1):1-4.
[24] 张慧霞,常晓悦,王利军. IL-6与肺部炎性疾病关系最新进展[J]. 国际呼吸杂志,2015,35(1):74-78.
[25] LITTLE C,O'BRIEN P J. An intracellular GSH-peroxidase with a lipid peroxide substrate[J]. Biochem Biophys Res Commun,1968,31(2):145-150.
[26] GENG N,WANG J,XU Y,et al. PM2.5 in an industrial district of Zhengzhou,China:Chemical composition and source apportionment[J]. Particuology,2013,11(1):99-109.
[27] 张岳. 痰热清注射液对脂多糖诱导大鼠急性肺损伤的保护作用[J]. 中医药临床杂志,2016,28(1):102-104.
[28] 颜娟,胡海娜,田嘉铭,等. 金莲花中总黄酮抗氧作用的研究[J]. 时珍国医国药,2010,21(2):386-387.
[29] 李楠,叶春华,吕艳蓓. 金莲花中黄酮类化合物抗氧化能力的研究[J]. 食品研究与开发,2010,31(11):77-79.
[30] 张书杰,张平,何平平,等. 急性肺损伤大鼠肺组织白细胞介素1β和肿瘤坏死因子α表达及黄芪的干预效应[J]. 中国组织工程研究与临床康复,2007,11(21):4112-4115.
[31] 刘一杰,薛永常. 植物黄酮类化合物的研究进展[J]. 中国生物工程杂志,2016,36(9):81-86.
[32] 杜云广,曹欣欣,王晓茹,等. 牡荆苷对脑缺血再灌注大鼠脑损伤保护作用及其机制研究[J]. 神经药理学报,2017,7(1):10-23.
[33] 方明月,李德利,刘双月,等. 基于分子对接技术的金莲花主要成分抗病毒和抗炎机制预测[J]. 中国中医药信息杂志,2018,25(7):57-61.
[34] 杨国栋,饶娜,田嘉铭,等. 金莲花中荭草苷和牡荆苷抗氧作用的研究[J]. 时珍国医国药,2011,22(9):2172-2173.

Effects of Trollius altaicus extract powder on PM2.5-induced lung injury in rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	LIU Mengmeng, LI Yanwen, ZHOU Qingbiao, ZHANG Jiaxin, WANG Lele, LIU Jiangzheng, YU Weihua, KONG Deqin, LIU Rui, HAI Chunxu, ZHANG Xiaodi. Effects of salidroside on mitochondria of lung in rats with chlorine-induced acute lung injury [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 31-36,41.
[2]	WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 379-382.
[3]	FAN Li, LIU Jianxiang, FENG Jiangbin, JIANG Xiaoyan, GAO Gang, TIAN Mei. Radioprotection effects from different extracts of Carthamus tinctorius [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 125-128,133.
[4]	LIU Mengmeng, KONG Deqin, ZOU Yuankang, WANG Lele, LIU Rui, HAI Chunxu, ZHANG Xiaodi. Protective effects of salidroside on chlorine-induced acute lung injury in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 377-382.
[5]	YANG Dong-hai1;ZHANG Tao;LIU Rui;LIU Jiang-zheng;QIN Xu-jun;WANG Zhao;ZHANG Wei;BAI Hua1;HAI Chun-xu;CHEN Hong-li. Ethyl pyruvate protects against chlorine gas -induced lung oxidative stress [J]. , 2012, 24(4): 253-255,.
[6]	GUO Guang-hua, YU Jing, CHEN Su-zuan, ZHANG Juan, LUO Li-li, XIE Li-hua, SU Zhong-jing, DONG Hong-mei, XU Hong, WU Li-biao. Antitumor Effect in Mice Induced by Vaccine of Esophageal Carcinoma Cell Line Fused with Cord Blood Dendritic Cells [J]. , 2007, 19(1): 11-015.