硒对邻苯二甲酸酯亚慢性暴露雄性大鼠肝脏的保护作用

doi:10.3969/j.issn.1004-616x.2018.06.012

Abstract

Abstract: OBJECTIVE: To investigate effects of sub-chronic exposure to diethylhexyl phthalate(DEHP) on livers of male rats and protective effect of selenium on the livers. METHODS: Seventy-seven male rats were randomly divided into 7 groups:control group (feeding basal feed),3 DEHP-treatment groups (diluted doses of 300,600 and 900 mg/kg,respectively),and 3 Selenium intervention groups (1 mg/kg yeast selenium was added to the corresponding dose of the exposure group). After 8 weeks of exposure,the rats were weighed,blood samples were collected for biochemical detection and the excised liver was weighed after 16 hours fasting. RESULTS: Compared with the control group,the mean weight of livers and coefficient of the liver were significantly increased in each of the exposed groups and the intervention group (P < 0.01). In addition,the quality of the 600 mg/kg treated group was significantly reduced (P < 0.05);the 900 mg/kg treated group was significantly lower in the body weight gain index (P < 0.01),and significantly lower than the control group in the total food intake and utilization index (P < 0.05);Compared with the treated group,the Se+300 mg/kg intervention group showed a significant decrease in weight gain (P < 0.05). The results of biochemical indicators showed that,compared with the control group,the 600 mg/kg treated group showed a significant increase in TP (P < 0.01);the 900 mg/kg treated group showed a significant increase in all five indicators (P < 0.01);The Se+300 mg/kg intervention group significantly increased the ALT index (P < 0.01),and significantly increased the ALT and AST indexes (P < 0.01). The Se+600 mg/kg intervention group significantly increased the ALT index (P < 0.05). There was a significant increase in AST index (P < 0.01). The Se+900 mg/kg intervention group showed significantly increased AST,TP and GLB (P < 0.01). Compared with the treated group,the intervention group was lower than the corresponding exposure group,and the Se+900mg/kg intervention group showed a significant decrease in TP and GLB indicators (P < 0.01). CONCLUSION: Sub-chronic exposure of DEHP affected body weight and growth,and liver function as well as caused liver swelling. The intervention of selenium effectively alleviated the toxic effect of DEHP on the liver.

Key words: diethylhexyl phthalate, rat, selenium, liver

CLC Number:

R994.6

NING Junkang, CAO Qian, YANG Mei, REN Xuedan, WANG Minsheng. Effects of selenium on livers of male rats with sub-chronic exposure of diethylhexyl phthalate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 479-482.

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Effects of selenium on livers of male rats with sub-chronic exposure of diethylhexyl phthalate

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