2,4-二氯苯氧乙酸对初断乳SD大鼠生殖器官发育的影响及相关机制

doi:10.3969/j.issn.1004-616x.2018.05.012

Abstract

Abstract: OBJECTIVE:To investigate reproductive toxicity of 2,4-dichlorophenoxyacetic acid (2,4-D) in juvenile male and female SD rats. METHODS:Male and female SD rats were exposed to different concentrations of 2, 4-D for 28 days (18.1,36.3 and 72.5 mg/kg),and to a placebo. The general behavior of animals,body weight changes and vaginal opening time were monitored during the period of exposure. After the exposure,the testis,epididymis, uterus,and ovary were weighed to calculate the wet weight and organ coefficient. In addition, blood serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T) and cholesterol levels were measured using an automatic biochemical analyzer. Pathological examinations were performed on hematoxylin-eosin (HE) stained rat testis,epididymis,ovary and uterus. RESULTS:Compared with the control group,the body weight gain of SD rats decreased significantly with increase of exposure doses (P < 0.05). The vaginal opening time of the SD rats in the 36.25 mg/kg group was significantly shortened while the vaginal opening time of the SD rats in the 72.5 mg/kg group was significantly prolonged (P < 0.05). The serum T levels from the 72.5 mg/kg group were decreased compared with the control group (P < 0.05). The cholesterol levels of female SD rats in the 72.5 mg/kg group were increased (P < 0.05). The serum E2 levels of female rats in the 36.25 mg/kg group increased significantly,while they were decreased in the 72.5 mg/kg group,and the differences were statistically significant (P < 0.05). The uterine cavity was larger and the uterine wall was thinner in the rats exposed to 72.5 mg/kg,and the number of spermatogenic cells in the epididymal tissues from the highdose group was reduced in the seminiferous tubules (layers). The uterine cavity was larger and the uterine wall was thinner in the rats exposed to 72.5 mg/kg,and the number of spermatogenic cells was reduced in the seminiferous tubules. CONCLUSION:Our study shows that 2,4-D interfered with development of reproductive system among young SD rats and the female rats were more affected than the males. A possible mechanism is that 2,4-D inhibited the synthesis of sex hormones by inhibiting the synthesis of testosterone from cholesterol,thereby affecting the development of reproductive systems.

Key words: 2,4-dichlorophenoxyacetic acid, reproductive system, developmental toxicity, sex hormone

CLC Number:

R994.6

GOU Lian, ZHANG Mengyun, LIU Keliang, PANG Dingguo, LIU Lida, XU Peiyu. Reproductive toxicity of 2,4-dichlorophenoxyacetic acid in immature SD rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 389-394.

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Reproductive toxicity of 2,4-dichlorophenoxyacetic acid in immature SD rats

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