协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2018.05.008

Abstract

Abstract: OBJECTIVE:To study expression of a costimulatory molecule, B7 homologue 4 (B7-H4), in precancerous lesions of human esophageal carcinoma and to understand its clinical significance in the formation of esophageal squamous cell carcinoma. METHODS:58 esophageal tissue specimens of endoscopic resection were collected. There were 16 cases of normal tissues,23 cases of low-grade intraepithelial neoplasia (LGIN) and 19 cases of high-grade intraepithelial neoplasia (HGIN). Hematoxylin eosin staining (HE) was used to evaluate their pathological changes. Expression of B7-H4 mRNA in esophageal tissues was detected by real-time fluorogenic quantitative-PCR (qPCR). Immunohistochemistry and Immunoblotting (Western blot) methods were used to detect B7-H4 protein expression in esophageal tissues. Expressions of IL-6, IL-10 and IFN-γ in esophagi were detected by Enzyme-linked Immunosorbent assay (ELISA). Additionally,correlations between expression of these cytokines and B7-H4 protein were analyzed. RESULTS:Immunohistochemistry and HE results indicate that B7-H4 protein expression was positively correlated with esophageal pathological grade (P < 0.01). Moreover,the qPCR results show that there was no significant difference in expression of B7-H4 mRNA between precancerous tissues and normal esophageal tissues (P > 0.05). Western blot data indicate that B7-H4 protein expression was increased significantly in precancerous esophagi compared with that of normal esophagi (P < 0.05). Furthermore,results of Western blot and ELISA tests show that B7-H4 expression was positively correlated with IL-6 concentration (P < 0.01). CONCLUSION:B7-H4 protein expression was gradually upregulated with the increase of precancerous grade of esophagi. B7-H4 may promote esophageal squamous cell carcinoma formation by interacting with IL-6.

Key words: esophageal squamous cell carcinmoma, B7-H4, esophageal precancerous conditions, IL-6

CLC Number:

R735.1

CHEN Xinran, SONG Yongzhi, SHAN Baoen. Clinical significance in expression of costimulatory molecule B7-H4 in precancerous esophageal tissues[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 368-373.

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Clinical significance in expression of costimulatory molecule B7-H4 in precancerous esophageal tissues

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