3种不同的HPV型别宫颈癌细胞株分泌蛋白非标记定量技术分析

doi:10.3969/j.issn.1004-616x.2018.05.005

Abstract

Abstract: OBJECTIVE:To analyze secretory proteins from three different kinds of cervical cancer cell lines (Siha, Caski and C33) and to identify specific proteins as biomarkers for earlier detection and for treatment. METHODS:Label-free quantitative proteomics technology processes were performed successively:protein cleavage, protein quantification and proteolysis. Then,the secretory proteins were analyzed and were picked out by LC-MS/MS and unlabeled Maxquant analyses. RESULTS:21 609 kinds of peptides and 2 895 different kinds of proteins were found. Meanwhile,collective proteins made up 29.2% of total with 729 different types. The numbers of proteins for Siha,Caski and C33 were 128,143 and 859,respectively. Among the collective proteins,there were 4 main protein species:heat shock proteins (HSPs)(14 proteins), heterogeneous nuclear ribonucleoprotein (HNRP)(8 proteins), laminin (LN)(4 proteins) and Aldo-keto reductase family 1(AKR1) (2 proteins). In addition,the lysosomal signaling pathway accounted for the largest proportion (39 proteins). CONCLUSION:There were 729 kinds of collective proteins among the three cervical cancer cell lines which belonged to the HPV16 type. Among the collective proteins,4 main protein species were concluded which have already been confirmed to play a significant role in the cervical cancer process. They may also belong to the lysosomal signaling pathway,The results may provide certain research directions for tumor molecular markers in the future.

Key words: cervical cancer, cell lines of cervical cancer, secretory protein, label-free analysis

CLC Number:

R730.3

HAO Yi, YUAN Jianlin, XIAO Yue, LI Hui, GUO Xia. Label-free quantitative proteomics analysis of secretory proteins from three different HPV-types of cervical cancer cell lines[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 354-358,364.

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Label-free quantitative proteomics analysis of secretory proteins from three different HPV-types of cervical cancer cell lines

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