食管鳞癌ECA109细胞中Survivin通过ERK信号通路调控c-myc基因表达的机制

doi:10.3969/j.issn.1004-616x.2018.05.003

Abstract

Abstract: OBJECTIVE:To study regulatory effects of Survivin on c-myc in esophageal ECA109 cells. METHODS:ECA109 esophageal squamous carcinoma cells were divided into Survivin shRNA interference,control shRNA and control groups (without any treatment). ECA109 cells were transfected with 2μg Survivin shRNA and 2μg control shRNA plasmids,respectively. After 48 h,expressions of Survivin,c-myc mRNA were detected by the RT-PCT method,proteins of Survivin,c-myc and p-ERK were detected by Western blot. ERK,p38,JNK,JAK/STAT3, PI3K/Akt signal pathway inhibitors were used to detect mRNA expression of c-myc by half quantitative RT-PCR and Western blot. RESULTS:Compared with the negative plasmid control group and the blank cell group,the expression of c-myc mRNA and protein was decreased after the interference of the Survivin shRNA group (P=0.022,P=0.000). c-myc mRNA and protein expressions were reduced in the ERK signal channel blockers group (P < 0.05). The expression of ERK phosphorylation protein was reduced after Survivin silence in the Survivin shRNA interference group. CONCLUSION:Survivin was effective in regulation of ERK activation state in upstream signal transduction. The role of Survivin in regulation of c-myc expression was related to the ERK signaling pathway.

Key words: shRNA, Survivin, c-myc, ERK

CLC Number:

R73-3

YAN Dong, WU Yixian, DONG Juanjuan, LI Xiumei, FEN Ming. Regulation of c-myc expression by Survivin via the ERK signal pathway in esophageal ECA109 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 345-348,353.

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Regulation of c-myc expression by Survivin via the ERK signal pathway in esophageal ECA109 cells

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