Carcinogenesis, Teratogenesis & Mutagenesis ›› 2018, Vol. 30 ›› Issue (4): 307-309,314.doi: 10.3969/j.issn.1004-616x.2018.04.012

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Effect of fine particle exposure on development of zebrafish embryos

WU Desheng1, WANG Honghui1, YUAN Jianhui1,2, WANG Hongju3, ZHAO Qionghui3, LIU Jianjun1   

  1. 1. Key Laboratory of Modern Toxicology of Shenzhen, Institute of Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055;
    2. Shenzhen Nanshan District Center for Disease Control and Prevention, Shenzhen 518054;
    3. Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518045, Guangdong, China
  • Received:2017-04-18 Revised:2018-04-23 Online:2018-07-30 Published:2018-07-30

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Abstract: OBJECTIVE: To investigate effect from exposure to fine particles on development of zebrafish embryos. METHODS: Zebrafish embryos were exposed to different concentrations (0,5,20,80,320 and 800 μg/mL) of fine particles for 96 h. Their survival and hatchability were observed under stereoscopic microscope at 0,2,4,6,8,16,24,48,72,96 h after the exposures. RESULTS: Compared with the control group,increased embryo mortality was observed in each of the treatment groups at 48 h after exposure,especially in the 80,320,800 μg/mL groups. Embryo hatchability was decreased with increased fine particle concentrations. Embryos did not hatched well in the 80 μg/mL group,and all embryos died in the 320,800 μg/mL groups at 48 h after exposure. Median lethal dose (LD50) of fine particles to zebrafish embryos was calculated to be (56.6±2.83) μg/mL. CONCLUSION: Exposure to fine particles caused death and hatching failure in zebrafish embryos.

Key words: fine particles, zebrafish, embryo development, air pollution

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