WFDC1基因在肝癌中的表达及其诊断和预后价值

doi:10.3969/j.issn.1004-616x.2018.04.009

Abstract

Abstract: OBJECTIVE: To investigate expression of the WFDC1 gene in hepatocellular tissues and cell lines and their clinicopathological values. METHODS: Expression of WFDC1 mRNA was detected using qPCR in tumor and adjacent tissues from patients with liver cancer,as well as in MC-LR-induced L02 malignantly transformed cells and in hepatocellular carcinoma cell lines. Expression in 331 cases of tumor tissues and 50 cases of adjacent tissues was analyzed based on the TCGA database. The relationship between expression of WFDC1 and survival and clinicopathological parameters was also studied. RESULTS: Results from the qPCR analyses show that expression of WFDC1 was significantly down-regulated in hepatocellular carcinoma tissues,MC-LR-induced L02 malignantly transformed cells and hepatocellular carcinoma cells (P < 0.05). The expression of WFDC1 in paracancerous tissues was significantly higher than that in tumor tissues in TCGA database (P < 0.01). Kaplan-Meier analysis show that patients with high expression of WFDC1 had longer survival time than those with low expression (Log-rank=4.80,P < 0.05). The results of ROC curve analysis show that the expression of WFDC1 was a specific and sensitive indicator of liver cancer (AUC=0.829). CONCLUSION: WFDC1 may be a tumor suppressor gene which can possibly provide reference value for diagnosis and survival prognosis of patients with liver cancer.

Key words: WFDC1 gene, hepatocellular carcinoma, gene expression, prognosis

CLC Number:

R730.2

ZHAO Ji, LIU Wenbin, CHEN Hongqiang, LIU Jinyi, YANG Huifang, CAO Jia. Expression of the WFDC1 gene in hepatocellular carcinoma tissues and cell lines and their prognostic values[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 291-296.

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Expression of the WFDC1 gene in hepatocellular carcinoma tissues and cell lines and their prognostic values

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