抗肿瘤新药乙烷硒啉对人舌鳞状细胞癌细胞增殖和迁移的影响

doi:10.3969/j.issn.1004-616x.2018.04.007

Abstract

Abstract: OBJECTIVE: To investigate the effect of a new type of organic selenium compound ethaselen (BBSKE) on growth and proliferation of a human tongue cancer CAL27 cell line. METHODS: In vitro cell assay MTT,Hoechst 33258 immunofluorescence staining,annexin V-FITC/PI staining flow cytometry,transwell chamber assay were performed to determine effects of 2,5,10,20 μmol/L (containing 1‰ DMSO) of BBSKE on CAL27 proliferation,and cell cycle and migration compared with solvent control and normal control groups. RESULTS: CAL27 cells treated with 5,10,and 20 μmol/L BBSKE had significantly reduced proliferation compared with untreated cultures (P < 0.01),and with further reduction in time. With the Hoechst 33258 immunofluorescence staining,the morphology of cells in the 5 and 10 μmol/L BBSKE groups was changed compared to the control group. Annexin V-FITC/PI staining flow cytometry results showed that the G2/M phase ratios in the 2,5,10,and 20 μmol/L BBSKE group were increased compared to the control group (P < 0.01),with blockage in the G2/M phase (P < 0.01). Results from the transwell chamber assay showed that the number of CAL27 cells exhibiting migration in the 2,5,10,and 20 μmol/L BBSKE groups were significantly lower than that in the control group (P < 0.01). The number of migrating cells in the 20 μmol/L BBSKE group was the least. In addition,the migration number and the concentration of drug were inversely proportional. CONCLUSION: BBSKE demonstrated significant inhibitory effect on CAL27 cells,induced cell apoptosis by arresting the cell cycle in the G2/M phase and inhibited cell migration. These observations suggest that BBSKE can be used as an alternative drug for chemotherapy in patients with TSCC.

Key words: ethaselen, thioredoxin, CAL27 cells, cell proliferation, cell cycle, apoptosis, cell migration

CLC Number:

XING Long, LI Yang, MA Xiaolong, MOHAMMED H, HUANG Ying, XIE Fuqiang, FENG Zhenghu. Effects of ethaselen, a novel organoselenium compound, on proliferation and migration of human tongue squamous carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 279-285.

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Effects of ethaselen, a novel organoselenium compound, on proliferation and migration of human tongue squamous carcinoma cells

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