淬灭胃癌SGC-7901细胞内活性氧对维生素E琥珀酸脂诱导内质网应激的影响

doi:10.3969/j.issn.1004-616x.2018.04.005

Abstract

Abstract: OBJECTIVE: To investigate effects of reactive oxygen species (ROS) scavenging on vitamin E succinate(VES)-induced endoplasmic reticulum stress response (ERS) in human gastric cancer SGC-7901 cells. MATERIALS: SGC-7901 human gastric cancer cells were treated with 1,5,10,20 mmol/L N-acetyl-L-cysteine (NAC) for 2 h and then with 20 μg/mL VES for an additional 12 h;ROS production was measured using confocal microscopy. In addition,cells were pretreated with 20 mmol/L NAC for 2 h and then with 20 μg/mL VES for an additional 12 h;there after,ROS production was measured using flow cytometry. In addition,cells were pretreated with 20 mmol/L NAC for 2 h and subsequently treated with 20 μg/mL VES for 15 h and 24 h,CHOP and GRP78 were determined by RT-PCR and Western blot. RESULTS: Pretreatment with NAC drastically decreased VES-induced ROS generation in SGC-7901 cells (P < 0.05). NAC significantly inhibited induction of CHOP and GRP78 mRNA and protein expression in VES-treated SGC-7901 cells (P < 0.05). CONCLUSION: ER stress response is an event downstream of the oxidative stress induced by VES in SGC-7901 cells.

Key words: reactive oxygen species, vitamin E succinate, endoplasmic reticulum stress, gastric cancer cells

CLC Number:

R730.231

HUANG Xiaoli, WU Kun, ZHAO Shasha. Reactive oxygen species scavenging on vitamin E succinate-induced endoplasmic reticulum stress response in SGC-7901 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 270-274.

References

[1] ZHAO Y,ZHAO X,YANG B,et al. Alpha-tocopheryl succinate-induced apoptosis in human gastric cancer cells is modulated by ERK1/2 and c-Jun N-terminal kinase in a biphasic manner[J]. Cancer Lett,2007,247(2):345-352.
[2] ZHAO Y,LI R,XIA W,et al. Bid integrates intrinsic and extrinsic signaling in apoptosis induced by alpha-tocopheryl succinate in human gastric carcinoma cells[J]. Cancer Lett,2010(1),288(1):42-49.
[3] JIA L,HUANG X L,ZHAO Y,et al. Vitamin E succinate (VES) inhibits cell growth and induces apoptosis by mitochondrial-derived ROS in SGC-7901 cells[J]. Med Sci Monit,2010,16(5):131-139.
[4] YIN ZEYUAN,ZHAO XUE,YANG DAN,et al. LFG-500,a newly synthesized flavonoid,induces apoptosis in human ovarian carcinoma SKOV3 cells with involvement of the reactive oxygen species-mitochondria pathway[J]. Exp Ther Med,2017,13(6):2819-2827.
[5] THANET S,WILAWAN M,YUKIO N,et al. Goniothalamin induces mitochondria-mediated apoptosis associated with endoplasmic reticulum stress-induced activation of JNK in HeLa cells[J]. Oncol Lett,2017,13(1):119-128.
[6] 黄晓莉,赵艳,张志宏,等. 维生素E琥珀酸酯通过内质网应激诱导胃癌SGC-7901细胞凋亡的研究[J]. 癌变·畸变·突变,2012,24(3):195-198.
[7] 黄晓莉,吴坤,赵莎莎. 维生素E琥珀酸脂诱导人胃癌细胞发生未折叠蛋白反应的研究[J]. 癌变·畸变·突变,2017,29(4):256-259.
[8] BREZNICEANU M L,LAU C J,GODIN N,et al. Reactive oxygen species promote caspase-12 expression and tubular apoptosis in diabetic nephropathy[J]. J Am Soc Nephrol,2010,21(6):943-954.
[9] LAMPIASI N,AZZOLINA A,D'ALESSANDRO N,et al. Antitumor effects of dehydroxymethylepoxyquinomicin,a novel nuclear factor-kappaB inhibitor,in human liver cancer cells are mediated through a reactive oxygen species-dependent mechanism[J]. Mol Pharmacol,2009,76(2):290-300.
[10] GUAN L,HAN B,LI Z,et al. Sodium selenite induces apoptosis by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction in human acute promyelocytic leukemia NB4 cells[J]. Apoptosis,2009,14(2):218-225.
[11] MOORADIAN A D,HAAS M J. Glucose-induced endoplasmic reticulum stress is independent of oxidative stress:A mechanistic explanation for the failure of antioxidant therapy in diabetes[J]. Free Radic Biol Med,2011,50(9):1140-1143.
[12] PIERACHILLE S,ANGELO C,PAOLO S,et al. Oxidative stress and respiratory system:pharmacological and clinical reappraisal of N-acetylcysteine[J]. COPD,2014,11(6):705-717.
[13] COOK K L,SOTO-PANTOJA D R,CLARKE P A G,et al. Endoplasmic reticulum stress protein GRP78 modulates lipid metabolism to control drug sensitivity and anti-tumor immunity in breast cancer[J]. Cancer Res,2016,76(19):5657-5670.
[14] KIM H,SHAHIN H,KOMUDI S,et al. Parkin regulation of CHOP modulates susceptibility to cardiac endoplasmic reticulum stress[J]. Sci Rep,2017,7:2093.
[15] KADARA H,LACROIX L,LOTAN D,et al. Induction of endoplasmic reticulum stress by the pro-apoptotic retinoid N-(4-hydroxyphenyl)retinamide via a reactive oxygen species-dependent mechanism in human head and neck cancer cells[J]. Cancer Biol Ther,2007,6(5):705-711.
[16] LEE Y J,SUH H N,HAN H J. Effect of BSA-induced ER stress on SGLT protein expression levels and alpha-MG uptake in renal proximal tubule cells[J]. Am J Physiol Renal Physiol,2009,296(6):F1405-1416.
[17] SHEIKH-ALI M,SULTAN S,ALAMIR A R,et al. Effects of antioxidants on glucose-induced oxidative stress and endoplasmic reticulum stress in endothelial cells[J]. Diabetes Res Clin Pract,2010,87(2):161-166.

Reactive oxygen species scavenging on vitamin E succinate-induced endoplasmic reticulum stress response in SGC-7901 cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Tingyu, CHEN Dayin, SHEN Hong, FU Xuyan, LU Chunfeng. Protective effects of quercetin on isoniazid-induced hepatotoxicity in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 12-16.
[2]	ZHAO Junwei, HUA Chenfeng, SHANG Pingping, XIE Fuwei, LI Xiang. Induction of oxidative stress by arsenious acid As(Ⅲ) in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 28-31.
[3]	SHI Pan, LIN Chonghua, SHU Yifang, QU Jiaquan, SONG Shuliang. Role of mitochondrial citrate transporter SLC25A1 in radiosensitivity of human esophageal squamous cell carcinoma in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 132-138.
[4]	JIN Lei, ZHOU Jiayou, HAN Jiacheng, GUAN Haojun, WANG Shuai, PENG Jie, WANG Xin, HAI Chunxu, LI Wenli. Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 21-28.
[5]	ZHANG Xuguang, DU Ruihu, CHEN Yanping, ZHAO Yan. Effect from activation of acid sphingomyelinase and ceramide on vitamin E succinate-induced apoptosis in human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 379-384.
[6]	ZHANG Zhiping, SHAN Baoen, SONG Jingjing, WANG Hao, LIU Yingzi, CHEN Wei, GAO Liping, LI Qiaoxia. Tumorigenic effect of interleukin?17 overexpressing gastric cells in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 295-299.
[7]	LI Ge, WANG Yaofei, GAN Na, HE Qi, WANG Guan, HAN Zhanfeng, HAI Chunxu, LIN Zhaoxing. Preliminary study on the role of MnSOD in liver regeneration [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 58-63,78.
[8]	ZHANG Jiaxin, LIU Ying, ZHANG Wei, ZHOU Qingbiao, KONG Deqin, LIU Rui, HAI Chunxu. Alteration of mitochondrial synthesis and function in epithelial-mesenchymal transition by TGF-β1 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 411-417.
[9]	ZHANG Chao, BAI Jianying, WANG Dongxin, YAN Dandan, WANG Pan, LIU Yanfei. Effects of formaldehyde on expression of endoplasmic reticulum stress-related Bip and CANX mRNA and proteins of HepG2 cell line [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 340-345,351.
[10]	HUANG Xiaoli, WU Kun, ZHAO Shasha. Vitamin E succinate induced unfolded protein response of human gastric cancer SGC-7901 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 256-259,265.
[11]	XU Peixiang, HOU Liying, SONG Huacui, YU Yang, WU Kun. Induction of autophagy and reactive oxygen by vitamin E succinate in human gastric carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 413-419.
[12]	CHEN Tingyu, SUN Jie, YANG Yu, ZHU Qiushuang, MIAO Zhi, ZHONG Tangwu, LU Chunfeng. The role of reactive oxygen species in isoniazid-induced DNA damage in L-02 cells and the protective effect of quercetin [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 472-476.
[13]	WANG Lele, LIU Jiangzheng, LIU Mengmeng, KONG Deqin, ZHANG Tao, YU Weihua, LIU Rui, ZHANG Xiaodi, WANG Xin, HAI Chunxu. Dose-dependent induction of renal injury by cadmium in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 446-452.
[14]	YU Yang, HOU Liying, SONG Huacui, WU Kun. Involvement of AMPK in vitamin E succinate-induced autophagy in human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 342-347.
[15]	ZHANG Yan, BAI Jianying, LEI Peiyu, WANG Youping, ZHAO Chunyan, LIANG Ruifeng. Effects of sodium sulfite on very low density lipoprotein assembly and secretion, and endoplasmic reticulum stress in HL-7702 liver cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 169-173,178.