Carcinogenesis, Teratogenesis & Mutagenesis ›› 2018, Vol. 30 ›› Issue (3): 230-233,238.doi: 10.3969/j.issn.1004-616x.2018.03.013

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Effects of Se on blood cell parameters of DEHP-exposed SD rats

YANG Mei1, CAO Qian1, NING Junkang1, REN Xuedan2, WANG Minsheng2   

  1. 1. School of Public Health of Southeast University, Nanjing 210009;
    2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, Jiangsu, China
  • Received:2018-03-13 Revised:2018-04-26 Online:2018-05-30 Published:2018-05-30

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Abstract: OBJECTIVE: To investigate the effects of different doses of di (2-ethylhexyl) phthalate (DEHP) on blood cell parameters in rats after 1 mg/kg selenium's intervention. METHODS: Seventy-seven healthy male SD rats were randomly divided into 7 groups:the control group,three DEHP groups with different concentrations (300,600,900 mg/kg),and Se +different doses of DEHP (1 mg/kg Se + 300,600,and 900 mg/kg DEHP). The rats were continuously fed for 90 days. During the experiment,the rats were observed for signs of poisoning (spiritual excitement,loose hair,partial hair loss). Body weight and whole blood were used to measure changes in various blood cell parameters at the end of the treatment time. RESULTS: Compared with the control group,the body weights of the rats exposed to 900 mg/kg DEHP were significantly decreased (P < 0.05). The proportion of toxic signs in the 600 and 900 mg/kg DEHP groups were 63.63% (7/11) and 100% (11/11),which were significantly higher than 0 (0/11) in the control group (P < 0.05). Among DEHP treated rats,the body weights were significantly increased after 1 mg/kg Se intervention (P < 0.05). The signs of poisoning in the 1 mg/kg Se+300 and 600 mg/kg DEHP groups were significantly lower. Compared with the control group,the WBC of the rats exposed to 600 and 900 mg/kg DEHP was significantly lower and the MONO was significantly higher (P < 0.05). Compared with the same dose of DEHP-treated group,the WBC of the 1 mg/kg Se+600 mg/kg DEHP group was significantly increased and the MONO was significantly decreased (P < 0.05). Among the erythrocyte parameters and compared with the control group,the MCH and HDW of the rats were significantly increased after exposure to DEHP;the RBC and HCT of the 600 and 900 mg/kg DEHP treated groups were significantly lower (P < 0.05). Compared with the same dose of DEHP-treated group,there was no significant difference in the red blood cell parameters of the rats after intervention by 1 mg/kg Se. Among the platelet parameters,no DEHP exposed group and Se intervention group had significant effects. CONCLUSION: Exposure of rats to 300-900 mg/kg DEHP affected certain red and white blood cell parameters. Intervention by selenium protected certain blood cell functions under the doses of 300-600 mg/kg DEHP.

Key words: selenium, di (2-ethylhexyl) phthalate, SD rat, blood cells

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