精子与肿瘤细胞融合模型的生物学特性研究

doi:10.3969/j.issn.1004-616x.2018.03.011

Abstract

Abstract: OBJECTIVE: To explore the biological characteristics of sperm-tumor cell fusion model. METHODS: A co-cultivation in vitro methods was used to study the time-effectiveness,cell inoculation density dependence and cell type specificity of the fusion model. The chimeric sperm-HeLa monoclonal cells were obtained by using micromanipulation and monoclonal culture method,which was then identified by using PCR test. The chimeric tumor cells in the cervical cancer paraffin were detected by amplification Y chromosome specific gene TSPY. RESULTS: Fusion between the sperm and tumor cells generally occurred in 1.5 hours after co-cultivation,the fusion rates was inversely proportional to the cell inoculation density,and the tumor cells who derived from an adenocarcinoma had a higher rate of cell fusion than other tumor cells and normal controls. One chimeric tumor tissue was observed in 20 cases of cervical cancer specimens,and one righteous mutation was found in TSPY gene. CONCLUSION: Cell fusion between the sperm and tumor cells displayed time-effectiveness,cell inoculation density dependence and cell type specificity. The chimeric tumor cells could be found in cervical cancer specimens.

Key words: sperm, tumor cells, cell fusion, chimeric cells

CLC Number:

Q279
Q944.47

CHEN Qiang, JIN Guangyi, LIN Guimiao, LIU Hao, XU Gaixia, WANG Xiaomei. Biological characteristics of a sperm-tumor cell fusion model[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 221-225.

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Biological characteristics of a sperm-tumor cell fusion model

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