QHF复方对人肝癌Huh7细胞生长、侵袭及肿瘤干细胞表面标志物表达的影响

doi:10.3969/j.issn.1004-616x.2018.03.002

Abstract

Abstract: OBJECTIVE: To explore the influence of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation,apoptosis and invasion and expression of surface markers of cancer stem cells. METHODS: Human Huh7 hepatoma cells were cultured in vitro. Cell proliferation inhibition rate was detected by MTT assay;cell cycle,apoptosis and cell surface marker CD133 and CD44 expression were detected by Flow cytometry;transwell experiment was used to observe cell invasion. RESULTS: QHF inhibited the proliferation of Huh7 cells,and there was a dose-dependent inhibitory effect. In addition,QHF promoted apoptosis and significantly increased cell proportion in S phase of the cell cycle. QHF lowered expression of tumor stem cell surface markers:CD133 and CD44. CONCLUSION: QHF was able to inhibit human Huh7 hepatoma cell proliferation and migration in vitro,the inhibition was related to the reduced expression of tumor stem cell surface markers:CD133 and CD44.

Key words: hepatocellular carcinoma, Chinese herbal compound QHF, cancer stem cells, CD133, CD44

CLC Number:

R730.1

LIU Dan, JIAO Liangbo, ZHANG Wen, WANG Xinyi, CHEN Tao, HU Wei. Effect of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation and invasion, and expression of surface markers of cancer stem cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 171-175.

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Effect of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation and invasion, and expression of surface markers of cancer stem cells

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