筛选化学过敏原的直接肽反应试验的建立

doi:10.3969/j.issn.1004-616x.2018.02.014

Abstract

Abstract: OBJECTIVE: To develop an in vitro alternative approach to the direct peptide reactivity assay (DPRA) for screening chemical allergens. METHODS: Lysine/cysteine peptides were incubated with test chemicals for 24 h and high performance liquid chromatography was performed. Depletion of the peptide was used as an indication of skin sensitization potential of chemicals. RESULTS: 12 chemicals representing allergens of different potencies caused depletion of greater than 6.38% with different reactivity,6 nonsensitizers gave depletion of less than 6.38%,which was consistent with LLNA sensitization classification. Unknown allergenic chloroform and benzene both yielded less than 6.38% depletion,which were judged as negative. CONCLUSION: The direct peptide reactivity assay appears to be a reliable predictor of sensitization potential for chemicals. In addition,it is simple,rapid and suitable for high throughput screening of chemical sensitization. Therefore,the assay can possibly be used for safety evaluation of chemicals.

Key words: peptide reactivity, allergens, skin sensitization, high performance liquid chromatograph

CLC Number:

R758.22

HU Peili, LIU Shibo, ZHANG Huiliang, LI Bo, WANG Gangli. Development of a peptide reactivity assay for screening chemical allergens'[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 150-154.

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Development of a peptide reactivity assay for screening chemical allergens'

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