Carcinogenesis, Teratogenesis & Mutagenesis ›› 2018, Vol. 30 ›› Issue (2): 114-119.doi: 10.3969/j.issn.1004-616x.2018.02.007

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Comparison of patient-derived non-small cell lung cancer xenegrafts models in NOD/SCID and nude mice

WU Jianbin1,2, JIANG Mei2, WANG Yue2, XU Jing2, YUE Wentao2,3, WANG Zitong1   

  1. 1. Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149;
    2. Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149;
    3. Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
  • Received:2017-07-03 Revised:2018-03-16 Online:2018-03-30 Published:2018-03-30

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Abstract: OBJECTIVE: To establish patient-derived non-small cell lung cancer xenegraft models in NOD/SCID or nude mice,and to compare the tumor-forming rates between these mice. METHODS: Non-small cell lung cancer tissues were collected from 23 patients who underwent surgical resection at the Department of Thoracic Surgery of Beijing Chest Hospital from July 2016 to December 2016. Non-small cell lung cancer tissues were subcutaneously implanted into NOD/SCID mice or BALB/c nude mice within 1 hour after surgical resection. Growth processes of the xenotransplanted tumors were observed,the tumor volumes were measured and the growth curves of the xenotransplanted tumors were plotted. The tumor-forming rates,the tumor latent time and the tumor formatting time were calculated. Then,comparison was made on morphology of patient-derived tumor xenegraft tissue and corresponding primary tumor tissue,and the related patients clinical pathological index of two groups,that could form xenegraft tumor group and couldn't form xenergraft tumor group. RESULTS: Xenegrafts were successfully established (55.6%) in NOD/SCID and (20%) nude mice. The tumor-forming rates of NOD/SCID mice models were higher than the nude mice models,and the tumor latent time (37 d,60 d,P=0.002) and the tumor formatting time (79 d,110 d,P=0.002) had significant differences. Tumor factors might associate with engraftment included squamous histology,TNM stage and the lymph node metastasis. Both mice models could keep the morphology of patient-derived tumor xenegraft tissues. CONCLUSION: The xenotransplantation models of patient-derived non-small cell lung cancer in both NOD/SCID mice and nude mice were successfully established. In comparison with the BALB/c nude mice model,the tumor-forming rates of the NOD/SCID mice model was higher,the tumor latent times and the tumor formatting times were shorter,suggesting that the NOD/SICD mice model was more suitable for the establishment of patient-derived NSCLC xenegraft model.

Key words: NSCLC, model, NOD/SCID mice, BALB/c nude mice

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