碳离子全身辐照致小鼠造血系统的损伤

doi:10.3969/j.issn.1004-616x.2018.02.006

Abstract

Abstract: OBJECTIVE: To explore the damage and recovery of hematopoietic system in mice after total body irradiation (TBI) with different doses of carbon-ion. METHODS: Female BABL/c mice were irradiated with 0.5,1,4,6 Gy carbon-ion beam. DNA damage in bone marrow monocytes cells (BMMCs) were detected by alkaline comet assay and cell cycle distributions were detected by FACS. Peripheral blood cell counts and spleen index were detected at 1^st,3^rd,8^th days after irradiation. RESULTS: Compared to the control group,carbon-ion TBI induced serious BMMNCs DNA damage in a dose-dependent manner (all r ≤ 0.96,P < 0.01). The most serious damage occurred at the 3^rd day post-irradiation. At the 8^th day after irradiation,DNA damage was still serious (P < 0.01). Carbon-ion TBI also caused dose-dependent bone marrow cells S phase and G2/M phase arrests and decrease of peripheral blood nucleated cells and spleen index. At the 8^th day after irradiation,the S phase arrests of bone marrow cells has been alleviated to some extent,but the decrease of peripheral blood nucleated cells and spleen index were still significant. CONCLUSION: Carbon-ion TBI caused serious damage to the hematopoietic system of mice in a dose-dependent manner. At the 8^th day after irradiation,the BMMCs DNA damage was still serious,and hematopoiesis did not recover.

Key words: carbon-ion irradiation, bone marrow cell, DNA damage and cell cycle distribution, peripheral blood cells, spleen index

CLC Number:

Q691.5

LIU Fang, WANG Zhuanzi, WEI Wei, LI Wenjian, DANG Bingrong. Total body irradiation of mice with carbon-ion caused damage to the hematopoietic system[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 109-113,119.

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Total body irradiation of mice with carbon-ion caused damage to the hematopoietic system

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