贝伐单抗动脉介入超选化疗对大鼠脑胶质瘤的影响

doi:10.3969/j.issn.1004-616x.2018.01.012

Abstract

Abstract: OBJECTIVE: To investigate the effectiveness of transcarotid artery chemotherapy on gliomas in rats. METHODS: After the brain glioma model was established successfully,rats were randomly divided into control group(the same amount of saline injected through the tail vein),intravenous injection of cisplatin group (V+CDDP),intravenous bevacizumab group (V+BVZ),arterial injection of cisplatin group (A+CDDP),arterial injection of bevacizumab group (A+BVZ). After treatment for 7 d,the glioma volume was detected,and the expression of VEGF,P21,Bcl-2 and MMP-2 protein and mRNA were detected by Western blot and real time-PCR. RESULTS: The glioma volume in A+CDDP group[(5.58±0.37) mm³] was smaller than that in V+CDDP group[(7.67±0.70) mm³]. The glioma volume in A+BVZ group[(4.81±0.20) mm³] was smaller than that in V+BVZ group[(6.57±0.69) mm³]. Differences were statistically significant (P < 0.01),and it was shown that artery injection had a strong inhibitory effect on the size of glioma. At the same time,the glioma volume in V+BVZ group was smaller than that in V+CDDP group (P < 0.01),the glioma volume in A+BVZ group was smaller than that in A+CDDP group (P < 0.05). BVZ had a stronger inhibitory effect on glioma size compared with CDDP,and artery injection had a stronger inhibitory effect than intravenous injection on the size of glioma. Compared with intravenous injection,the expression of VEGF,Bcl-2 and MMP-2 protein and mRNA were significantly decreased (P < 0.05) and the expression of P21 protein and mRNA was significantly increased in arterial injection group (P < 0.05). Compared with CDDP,the expression of VEGF,Bcl-2 and MMP-2 protein and mRNA were significantly decreased (P < 0.05) and the expression of P21 protein and mRNA was significantly increased (P < 0.05) in BVZ group. CONCLUSION: Application of BVZ transcatheter arterial chemoinfusion effectively inhibited the size of glioma,therefore,this approach may be useful for providing better therapeutic effect.

Key words: glioma, transcatheter arterial chemoinfusion, bevacizumab

CLC Number:

R730.5

GAO Fei, WANG Yingbin. Experimental transcarotid artery chemotherapy on gliomas[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 62-66.

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Experimental transcarotid artery chemotherapy on gliomas

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