HIF-1α高表达对苯代谢物诱导K562细胞毒性的影响

doi:10.3969/j.issn.1004-616x.2018.01.009

Abstract

Abstract: OBJECTIVE: To study the effects of expression of HIF-1α on cytotoxicity of K562 cells induced by 1,4-benzoquinone (1,4-BQ),hydroquinone (HQ) and phenol. METHODS: The control K562 cells (K-c) and K562 cells with high expression of HIF-1α (K-h) were treated with different concentrations of 1,4-BQ (0,10,20,40,80 μmol/L),HQ (0,10,20,40,80 μmol/L) and phenol (0,1,1.5,2,2.5,5 mmol/L) for 24 h. Cell proliferation was detected by MTT assay,apoptosis was detected by flow cytometry with PI/FITC Annxein V double staining,and cell cycle was detected by propidium iodide combined with flow cytometry. RESULTS: The MTT results revealed that the relative proliferation rate of K-c cells and K-h cells showed a dose-related decrease after treating with 1,4-BQ (P < 0.05). The relative proliferation rate of K-h was higher than control K-c (P < 0.05) in 80 μmol/L 1,4-BQ. After treating with HQ and phenol at higher concentrations,K-c exhibited proliferation inhibition,while no significant proliferation inhibition was observed in K-h. Flow cytometry results revealed that the apoptosis rate increased in K-c and K-h (P < 0.05) and showed a dose-response relationship after exposuring to 1,4-BQ for 24 h (P < 0.05). Moreover,the apoptosis rate of K-hwas lower than K-c (P < 0.05) with 20 μmol/L 1,4-BQ. After exposure to HQ and phenol,K-c and K-h demonstrated no significant differences compared with control group,K-h was not significantly different from K-c. The results of cell cycle analysis demonstrated K-c were arrested in G0/G1 phase or S-phase after exposure to 1,4-BQ,HQ and phenol. CONCLUSION: Among benzene metabolites,1,4-BQ was the most cytotoxic,followed by HQ and phenol. High expression of HIF-1α reduced the cytotoxicity of K562 cell caused by benzene metabolites,and HIF-1α might work in regulating cell proliferation,apoptosis through cell cycle control.

Key words: HIF-1α, benzene metabolites, proliferation, apoptosis, cell cycle

CLC Number:

R994.6

SUN Fengxia, MENG Xing, SUN Rongli, ZHANG Juan, YIN Lihong, PU Yuepu. Relationship between expression of HIF-1α and cytotoxicity from exposure to benzene metabolites on K562 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 47-51,57.

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Relationship between expression of HIF-1α and cytotoxicity from exposure to benzene metabolites on K562 cells

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