辐射诱发大鼠体内旁效应中肺及肾组织凋亡相关基因mRNA的表达变化

doi:10.3969/j.issn.1004-616x.2018.01.008

Abstract

Abstract: OBJECTIVE: To investigate expression of apoptosis-related genes during different dose of X-ray-induced bystander effects in rats. METHODS: 70 male Wistar rats were randomly divided into the normal control group and the different irradiation groups. For the latter groups,the right lungs of the rats were radiated with 2,4,8 Gy X-rays. During irradiation,the rest of the body was covered with lead shielding. At 6,24 and 48 h respectively after radiation,7 rats of each radiation group were executed. Variations in mRNA expression of Cytochrome C,Caspase-9,Caspase-3,Bax and Bcl-2 in the right lung,left lung and left kidney tissue were detected with quantitative polymerase chain reaction (qPCR). RESULTS: The mRNA expressions of Cytochrome C,Caspase-9 and Caspase-3 in the right lung,left lung and left kidney tissue of the irradiated groups were up-regulated at all time points. However,the mRNA expressions of Bcl-2 were down-regulated at all time points in the right and left lungs. The observed changes were significantly different in comparison with the normal control group (P < 0.05 or P < 0.01). In the right lung,expressions of Bax mRNA were significantly increased in all irradiated groups except the 8 Gy dose group at 48 h (P < 0.05 or P < 0.01). In the left lung,expressions of Bax mRNA were significantly increased in each radiation group (P < 0.01). In the left kidney,expressions of Bax mRNA were significantly increased in all irradiated groups except the 2 Gy dose group at 6 h. Compared with the normal control group,the differences were statistically significant (P < 0.01). Expressions of Bcl-2 mRNA were decreased at all time points after irradiation except the 8 Gy dose group at 6 h and the difference was statistically significant compared with the normal control group (P < 0.05 or P < 0.01). CONCLUSION: After the right lungs of the rats were irradiated by different doses of X-rays,expressions of Cytochrome C,Caspase-9,Caspase-3 and Bax in the right lung tissue were up-regulated. While expressions of Bcl-2 were down-regulated. The left lung and left kidney tissues that were not irradiated also responded to the X-rays. And the radiation-induced bystander effects were observed in vivo. Between every radiation dose and every time point,there were no apparently different effects.

Key words: radiation induced bystander effect, apoptosis-related genes, lung and kidney tissue, mRNA expression

CLC Number:

R852.7

ZHANG Chaoning, LI Jintian, LIU Yongqi, LIN Xingyao. Expression of apoptosis-related genes in lung and kidney tissues of rats with radiation-induced bystander effects[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 42-46.

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Expression of apoptosis-related genes in lung and kidney tissues of rats with radiation-induced bystander effects

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