氯气吸入致大鼠肺血管内皮细胞的线粒体损伤及红景天苷的干预作用

doi:10.3969/j.issn.1004-616x.2018.01.006

Abstract

Abstract: OBJECTIVE: To observe the effects of chlorine on structure and function of mitochondria of lungs in rats and explore the protective effects of salidroside on acute lung injury induced by chlorine. METHODS: 36 healthy male Sprague-Dawley (SD) rats were divided into 6 groups:1 control and 5 exposure. Rats in the exposure groups were exposed to an initial concentration of 1 200 mg/m³ chlorine for 5 min in a devised cabinet or room air. Rats were sacrificed at 0.5,1.5,3,6 and 9 h,respectively,after exposure. In addition,24 rats were randomly divided into 4 groups:control,chlorine,chlorine+salidroside and salidroside. Chlorine and chlorine+salidroside groups were exposed to chlorine as mentioned above. Chlorine+salidroside and salidroside groups were treated with salidroside (300 mg/kg) at 30 min before chlorine exposure and at 15 min after chlorine exposure. Then the rats were sacrificed at 3 h after chlorine exposure,and the lung tissues were harvested. Lung mitochondria ultra structures were observed with electron microscope. The Na⁺,K⁺-ATPase,Ca²⁺,Mg²⁺-ATPase in lung tissues and lactate dehydrogenase (LDH) in serum and bronchoalveolar lavage fluid (BALF) were measured using kits,respectively. The mitochondria in lung tissues were isolated with differential centrifugation. The protein levels of Tom 20,PINK1 and Parkin in lung tissues or mitochondria were detected by western blotting. RESULTS: Compared with control group,LDH of BALF in exposure groups were significantly increased (P < 0.05). Tom 20,PINK1 and Parkin protein expressions in lung tissues were all higher than that in control group (P < 0.05). Expression of Tom 20 was highest at 3 h. It was also shown by transmission electron microscope that the mitochondria structure in the control group was normal. The mitochondria in rat pulmonary vascular endothelial cells were swollen with disrupted or disintergrated cristae. When treated with salidroside,the expression levels of PINK1 and Parkin in lung tissues and PINK1 in mitochondria were reduced compared with chlorine group at 3 h. The expressions of Parkin in mitochondria was markedly increased (P < 0.05);Na⁺,K⁺-ATPase,Ca²⁺,Mg²⁺-ATPase in lung tissues and LDH in serum were decreased. CONCLUSION: Chlorine induced mitochondrial structural damage and functional impairment in rats and salidroside exerted a beneficial effect through protecting the mitochondria.

Key words: chlorine, mitochondria, acute lung injury, salidroside

CLC Number:

R961

LIU Mengmeng, LI Yanwen, ZHOU Qingbiao, ZHANG Jiaxin, WANG Lele, LIU Jiangzheng, YU Weihua, KONG Deqin, LIU Rui, HAI Chunxu, ZHANG Xiaodi. Effects of salidroside on mitochondria of lung in rats with chlorine-induced acute lung injury[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 31-36,41.

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Effects of salidroside on mitochondria of lung in rats with chlorine-induced acute lung injury

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