大蒜素对顺铂肾毒性的保护作用

doi:10.3969/j.issn.1004-616x.2018.01.004

Abstract

Abstract: OBJECTIVE: To investigate the protective effect of allicin on cisplatin (DDP)-induced oxidative damage in HEK293 cells. METHODS: HEK293 cells were cultured in vitro and organized into different experimental groups:control,DDP treatment,allicin treatment,DDP+allicin treatment. Cell survival and expression of cytotoxicity were determined by using the MTT method. Superoxide dismutase (SOD) activity was examined by the xanthine oxidase method,malondialdehyde (MDA) content by the thiobarbituric acid method and glutathione (GSH) content by the nitrobenzoic acid method. RESULTS: Cell survival rate decreased gradually when treated with DDP alone,and this effect was dose-dependent. After pretreatment with catechin and then with DDP (20 mg/L),the cell survival rate improved significantly (P < 0.01). Allicin treatment significantly increased the intracellular MDA content,but decreased the SOD activity and GSH level compared with that in the DDP control group (P < 0.01). CONCLUSION: Allicin can antagonize DDP-induced oxidative damage in HEK293 cells and has obvious protective effects on DDP-induced toxicity in kidney cells.

Key words: allicin, cisplatin, nephrotoxicity, antioxidant

CLC Number:

R994.1

HAN Hedan, WANG Hai, GAO Liping. Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 20-24.

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Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells

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