微小RNA-202对A549细胞增殖和周期的作用及机制

doi:10.3969/j.issn.1004-616x.2017.06.010

Abstract

Abstract: OBJECTIVE:To investigate the regulatory mechanism on proliferation of miR-202 in A549 lung cancer cells. METHODS:Through the lentivirus transfection into A549 cells,up-and down-expression of miR-202 were investigated. DIANA-TOOLs and KEGG database were used to analyze the bioinformatic of miR-202 expression and to explore the signaling pathways that might be involved with potential target genes. Then,MTT assay and flow cytometry technology were applied to detect the influence of miR-202 on cell proliferation,apoptosis and cycle. RT-qPCR and Western blot methods were applied to detect the miR-202 regulation for its predicted target gene on both mRNA and protein levels. RESULTS:Bioinformatics analysis show that,miR-202 was involved in non-small cell lung cancer and other tumor-related pathways,and possibly in regulation of PIK3CA. Functional studies show that,compared with the negative control group,up-regulation of miR-202 inhibited cell proliferation (P < 0.05),caused cell cycle G1/S phase arrest (P < 0.05),but did not affect cell apoptosis (P > 0.05). Target gene study show that,compared with the control group,up-regulation of miR-202 reduced the expression of PIK3CA protein (P < 0.05),but had no effect on the expression of PIK3CA mRNA(P > 0.05). CONCLUSION:Expression of miR-202 influenced the proliferation and cycle of A549 cells through the negative regulation of PIK3CA and the study provides a new clue to the role and mechanism of miRNA in lung cancer.

Key words: Lung cancer, miR-202, cell proliferation, cell cycle, regulatory mechanism

CLC Number:

R730.2

PENG Hui, MA Shumei, HONG Weiwei, SUI Jing, ZHANG Yanqiu, LIANG Geyu. Regulatory mechanism of miR-202 on proliferation of A549 lung cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 454-459.

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Regulatory mechanism of miR-202 on proliferation of A549 lung cancer cells

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