GMA诱导16HBE恶性转化细胞中LncRNA EMX2OS的表达及意义

doi:10.3969/j.issn.1004-616x.2017.06.004

Abstract

Abstract: OBJECTIVE:To investigate the expression of long non-coding RNA EMX2OS (LncRNA EMX2OS) during glycidyl methacrylate (GMA)-induced malignant transformation of 16HBE cells. METHODS:Malignantly transformed 16HBE cells (induced by exposure to 8 μg/mL GMA) and solvent control cells (exposed to DMSO) at around the 30th generation were harvested. High throughput LncRNA microarrays were used to detect differences in expression profile of LncRNAs between the two groups of cells. Changes in LncRNAs were screened through strategies such as fold changes and neighboring genes analyses. Real-time fluorescence quantitative PCR (qPCR) and gene chip were applied to measure the expression levels of LncRNA EMX2OS and EMX2,respectively. RESULTS:Based on the result of LncRNA microarrays,expressions of LncRNA EMX2OS was up-regulated by 6.01 fold in the transformed cells campared to the control cells. In addition, qPCR analyses showed that LncRNA EMX2OS increased 3.37 fold and EMX2 increased 1.88 fold change (P < 0.05). The trends of increase in EMX2 and EMX2OS trends were identical. CONCLUSION:GMA induced significant increase in expression of LncRNA EMX2OS of 16HBE cells and the expression can be considered as a specific biomarker which is involved in GMA-induced malignant transformation 16HBE cells.

Key words: glycidyl methacrylate, human bronchial epithelial cells, LncRNA EMX2OS, EMX2

CLC Number:

R730.2

WANG Quankai, WANG Boshen, XIE Guangyun, KANG Tongying, ZHU Baoli, XU Jianning. Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 422-426.

References

[1] POLLEUX F. Generation of the cortical area map;emx2 strikes back[J]. Neuron,2004,43(3):295-297.
[2] CECCHI C. Emx2:a gene responsible for cortical development, regionalization and area specification[J]. Gene,2002,291(1/2):1-9.
[3] QIU H,YAN Q,LUO X,et al. EMX2 is downregulated in endometrial cancer and correlated with tumor progression[J]. Int J Gynecol Pathol,2013,32(2):193-198.
[4] LI J,MO M,CHEN Z,et al. Adenoviral delivery of the EMX2 gene suppresses growth in human gastric cancer[J]. PLoS One,2012,7(9):e45970.
[5] YUE D,LI H,CHE J,et al. EMX2 is a predictive marker for adjuvant chemotherapy in lung squamous cell carcinomas[J]. PLoS One,2015,10(7):e132134.
[6] GIROUX L E,HIRATA T,MO M,et al. The homeobox gene EMX2 is a prognostic and predictive marker in malignant pleural mesothelioma[J]. Lung Cancer,2014,85(3):465-471.
[7] NOONAN F C,GOODFELLOW P J,STALOCH L J,et al. Antisense transcripts at the EMX2 locus in human and mouse[J]. Genomics,2003,81(1):58-66.
[8] 阳艳,章汉旺. EMX2、HOXA10与子宫内膜容受性[J]. 生殖与避孕,2006(2):95-98.
[9] TAKAMOCHI K,OH S,MATSUOKA J,et al. Clonality status of multifocal lung adenocarcinomas based on the mutation patterns of EGFR and K-ras[J]. Lung Cancer,2012,75(3):313-320.
[10] 杨敏,许建宁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化研究[J]. 中华预防医学杂志,2009,43(3):187-192.
[11] 董琳,胡洁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化过程中相关基因表达变化的研究[J]. 癌变·畸变·突变,2010,22(4):249-254.
[12] LI K,BLUM Y,VERMA A,et al. A noncoding antisense RNA in tie-1 locus regulates tie-1 function in vivo[J]. Blood,2010,115(1):133-139.
[13] LIU T,HUANG Y,CHEN J,et al. Attenuated ability of BACE1 to cleave the amyloid precursor protein via silencing long noncoding RNA BACE1AS expression[J]. Mol Med Rep,2014,10(3):1275-1281.
[14] FAGHIHI M A,MODARRESI F,KHALIL A M,et al. Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase[J]. Nat Med,2008,14(7):723-730.
[15] TRAN V G,COURT F,DUPUTIE A,et al. H19 antisense RNA can up-regulate Igf2 transcription by activation of a novel promoter in mouse myoblasts[J]. PLoS One,2012,7(5):e37923.
[16] SPIGONI G,GEDRESSI C,MALLAMACI A. Regulation of Emx2 expression by antisense transcripts in murine cortico-cerebral precursors[J]. PLoS One,2010,5(1):e8658.
[17] 周吉,沈聪,霍然. Emx2和Emx2OS在小鼠胚胎生殖嵴中的表达[J]. 生殖与避孕,2014(6):437-443.
[18] XU C,YANG M,TIAN J,et al. MALAT-1:a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis[J]. Int J Oncol,2011,39(1):169-175.
[19] FRAMPTON A E,CASTELLANO L,COLOMBO T,et al. MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression[J]. Gastroenterology,2014,146(1):268-277.
[20] YU T,BACHMAN J,LAI Z C. Evidence for a tumor suppressor role for the large tumor suppressor genes LATS1 and LATS2 in human cancer[J]. Genetics,2013,195(3):1193-1196.
[21] SOH U J,LOW B C. BNIP2 extra long inhibits RhoA and cellular transformation by Lbc RhoGEF via its BCH domain[J]. J Cell Sci,2008,121(Sup 10):1739-1749.
[22] YUE D,LI H,CHE J,et al. EMX2 Is a Predictive marker for adjuvant chemotherapy in lung squamous cell carcinomas[J]. PLoS One,2015,10(7):e132134.

Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells

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