微囊藻毒素-LR对人正常食管上皮细胞凋亡及对Caspase-3和Caspase-9蛋白表达的影响

doi:10.3969/j.issn.1004-616x.2017.06.003

Abstract

Abstract: OBJECTIVE:To investigate the effects of microcystin-LR on viability and apoptosis of human normal esophageal epithelial cells,and on expression of Caspase-3 and Caspase-9. METHODS:Human normal esophageal epithelial cells were treated with microcystin-LR at different concentrations (1,3.75,7.5,15,30,50 μg/mL) for 24 h,and cell viability was detected by the MTT assay. The data were used to calculate the half inhibitory concentration (IC₅₀) which was used to determine the MC-LR concentrations for subsequent experiments. Accordingly,cells were treated with 6,12 and 24 μg/mL MC-LR,and apoptosis was detected by PI-Annexin V double staining. Expression levels of Caspase-3 and Caspase-9 were detected by Western blot. RESULTS:Compared with the control group,microcystin-LR of 3.75-50 μg/mL decreased the viability of normal esophageal epithelial cells (P < 0.01);the 6-24 μg/mL concentrations increased the apoptosis rate (P < 0.05 or P < 0.01),and the expression of Caspase-3 and Caspase-9 (P < 0.01). CONCLUSION:Induction of apoptosis in human normal esophageal epithelial cells by microcystin-LR may be related to its activation of Caspase-3 and Caspase-9.

Key words: microcystin-LR, human esophageal epithelial cells, apoptosis, Caspase-3, Caspase-9

CLC Number:

R364

XIAO Hongmei, WU Yi, ZHANG Ting, WANG Shaokang, SUN Guiju. Effects of microcystin-LR on apoptosis, and Caspase-3 and Caspase-9 expression in human normal esophageal epithelial cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 418-421,426.

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Effects of microcystin-LR on apoptosis, and Caspase-3 and Caspase-9 expression in human normal esophageal epithelial cells

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