人尿源性干细胞的原代培养

doi:10.3969/j.issn.1004-616x.2017.05.014

Abstract

Abstract: OBJECTIVE: To establish a method for isolation,cultivation and preservation of human urine-derived stem cells(hUSCs). METHODS: hUSCs were isolated from fresh urine samples from adult healthy volunteers. Survival rate of hUSCs after cryopreservation and resuscitation was detected by trypan blue staining to determine the best formula for freezing medium. Proliferation of hUSCs was determined by MTT assay. Stem cell surface marks were detected by flow cytometry. RESULTS: 6/10 hUSCs were successfully isolated and cultured from urine. The survival rate of hUSCs in the freezing medium-DMSO:FBS:hUSCs medium=1:4:5-was the highest based on trypan blue staining. MTT assay showed that the growth curve of hUSCs was S-shaped showing good proliferation activity. Mesenchymal stem cell surface marker CD44 and CD90 were detected positively using flow cytometry. CONCLUSION: A method for isolation,cultivation and preservation of human urine-derived stem cells was successfully established.

Key words: human urine-derived stem cells, primary culture, CD44, CD90

CLC Number:

Q813.1+1

ZHOU Ming, SUN Zhenxiao. Cultivation of human urine-derived stem cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 390-393.

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Cultivation of human urine-derived stem cells

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