Carcinogenesis, Teratogenesis & Mutagenesis ›› 2017, Vol. 29 ›› Issue (5): 387-389,393.doi: 10.3969/j.issn.1004-616x.2017.05.013

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Transient expression of HBx after hydrodynamic gene delivery in mice

HUANG Danmei1, WU Manpeng2, HUANG Xiaohua1, ZHANG Ying1, NI Yangpeng3, NIU Yongdong1   

  1. 1. Department of Pharmacology, Shantou University Medical College, Shantou 515041;
    2. Department of Pediatrics, The Second People's Hospital of Shantou, Shantou 515011;
    3. Department of Pathology, People's Hospital of Jieyang, Jieyang 522091, Guangdong, China
  • Received:2017-01-10 Revised:2017-05-27 Online:2017-09-30 Published:2017-09-30

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Abstract: OBJECTIVE: To establish a transiently expressed HBx via hydrodynamic gene delivery in mice. METHODS: Two experimental groups of CD-1 male mice (4 mice per group) were injected with pcDNA-Flag-HBx plasmid or pcDNA empty vector via their tail veins. Mice were then sacrificed and their liver tissues were collected for extraction of RNA and protein. RESULTS: HBx was detected by PCR and Western blotting at the RNA and protein levels,respectively. Transiently expressed HBx effectively regulated the expression of typical target genes,small heterodimer partner (SHP) and cytochrome P450 7A1(Cyp7a1) of farnesoid X receptor signaling,as shown by real-time PCR analyses. CONCLUSION: A transient expression model of HBx via hydrodynamic gene delivery in mice was established.

Key words: hydrodynamic, transient gene expression, tail vein injection, HBx

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