苯并芘诱导肝脏的炎症损伤及EGFR的表达变化

doi:10.3969/j.issn.1004-616x.2017.05.012

Abstract

Abstract: OBJECTIVE: To investigate the effect of long-term benzopyrene (BaP) exposure on expression of epithelial growth factor receptor(EGFR) in SD rat liver. METHODS: 36 healthy SD female rats were randomly divided into exposure and control groups with 18 rats per group. Rats in the exposure groups were injected with 0.2 mL of 10 mg/mL corn-benzopyrene through chest punctured,once per 2 weeks for 2 months. Rats in the control group were injected with 0.2 mL corn oil. All rats were observed the survival status and body mass changes for one year. Immunohistochemical,immunofluorescence and Western blot assays were used to detect expression of EGFR protein in livers and HE staining for pathological effects. RESULTS: HE staining showed significant fragmentation necrosis and inflammatory infiltration after benzopyrene exposure. Immunohistochemistry and immunofluorescence showed that expression of EGFR in the liver of the exposure group was significantly higher than that of the control group. Based on Western blot results (0.452±0.095,0.915±0.253), expression level of EGFR protein in the exposed group was significantly higher than that in the control group (P < 0.05). CONCLUSION: BaP induced hepatic inflammation and EGFR overexpression,suggesting that the two effects were closely related.

Key words: benzopyrene, liver injury, inflammation, epithelial growth factor receptor

CLC Number:

R994.6

DENG Yanxia, GU Pan, LIU Hailong, ZHENG Kai, LIU Jianjun, HUANG Xinfeng, LI Xiyi, HUANG Haiyan. Expression of EGFR in benzopyrene-induced hepatic inflammation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 383-386.

References

[1] 张晶. 苯并(a)芘致小鼠肝细胞凋亡及相关机制的研究[D]. 吉林:吉林大学,2005.
[2] 尼佳乐. 维生素E对苯并[a]芘诱导雄性大鼠的肝脏毒性的保护作用[D]. 四川:重庆医科大学,2015.
[3] 顾其华,胡成平,夏莹,等. 3,4-苯并芘肺内注射构建大鼠肺肿瘤模型的实验研究[J]. 中国肺癌杂志,2007,10(3):172-175.
[4] CIARDIELLO F,TORTORA G. A novel approach in the treatment of cancer:targeting the epidermal growth factor receptor[J]. Clin Cancer Res,2001,7(10):2958-2970.
[5] 王一,黎燕,沈倍奋,等. 炎症:肿瘤的推进器[J]. 国际药学研究杂志,2014,41(1):63-67.
[6] MA J Q,LIU C M,QIN Z H,et al. Ganodermaapplanatum terpenes protect mouse liver against benzo(alpha) pyren-induced oxidative stress and inflammation[J]. Environ Toxicol Pharmacol, 2011,31(3):460-468.
[7] SCHLESSINGER J. Ligand-induced,receptor-mediated dimerization and activation of EGF receptor[J]. Cell,2002, 110(6):669-672.
[8] SISTO M,LISI S,D'AMORE M,et al. The metalloproteinase ADAM17 and the epidermal growth factor receptor (EGFR) signaling drive the inflammatory epithelial response in Sjogren's syndrome[J]. Clin Exp Med,2015,15(2):215-225.
[9] CARVER R S,STEVENSON M C,SCHEVING L A,et al. Diverse expression of ErbB receptor proteins during rat liver development and regeneration[J]. Gastroenterology,2002, 123(6):2017-2027.
[10] WANG T,TAKIKAWA Y,TABUCHI T,et al. Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway[J]. J Gastroenterol,2012,47(7):805-813.
[11] BERASAIN C,AVILA MA. The EGFR signalling system in the liver:from hepatoprotection to hepatocarcinogenesis[J]. J Gastroenterol,2014,49(1):9-23.
[12] 王李杰. 炎症促进肝细胞癌进展和转移作用机制的体内和体外研究[D]. 北京:中国人民解放军军医进修学院,2011.

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[15]	ZHANG Mei, LI Hui, LUO Hong-jun, LUO Wen-hong. Changes of Semicarbazide-sensitive Amine Oxidase(SSAO) Activity in Pulmonary Inflammation Induced by Lipopolysaccharide [J]. , 2007, 19(3): 223-227.

Expression of EGFR in benzopyrene-induced hepatic inflammation

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