PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响

doi:10.3969/j.issn.1004-616x.2017.05.011

Abstract

Abstract: OBJECTIVE: To explore effects of PM2.5 exposure on expression of oxidative stress and DNA methylation genes during zebrafish embryo development. METHODS: Samples of PM2.5 were collected by large-volume samplers on fiber filters. Then,the particle materials were collected by ultrasonicing and freeze-drying. Zebrafish embryos were exposed to PM2.5 particle materials in the concentrations of 0,5 and 20 μg/mL. After extraction of total RNA,relative expression of oxidative stress genes (sod1 and ogg1) and DNA methylation genes (tet1 and dnmt1) was detected by qPCR. RESULTS: 2-6 h after PM2.5 exposure,expression of sod1,tet1 and dnmt1 were increased significantly with a dose-dependent relationship (r=0.98,0.98,0.99,P < 0.05,respectively);2-6 h after PM2.5 exposure,expression of ogg1 was increased significantly in the 5 μg/mL group (P < 0.05);24-48 h after PM2.5 exposure,expression of sod1,ogg1,tet1 and dnmt1 were decreased significantly compared with those in early stage (2-6 h). CONCLUSION: Expression of oxidative stress genes (sod1 and ogg1) and DNA methylation genes (tet1 and dnmt1) could be affected from exposure to PM2.5,especially during early embryo development.

Key words: PM2.5, embryo development, oxidative stress, DNA methylation

CLC Number:

R994.6

WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 379-382.

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Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos

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