纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究

doi:10.3969/j.issn.1004-616x.2017.05.010

Abstract

Abstract: OBJECTIVE: To study the adverse effect of nano-SiO₂ particles on reproductive system in male SD rats. METHODS: Male rats were treated with nano-SiO₂ particles or saline and then executed 4 weeks later. Testes were isolated for the extraction of protein. 2D-DIGE coupled with MALDI-TOF-MS was used to identify nano-SiO₂ induced abnormal expression of proteins. Two proteins were verified by Western blot analysis. RESULTS: Nano-SiO₂ induced abnormal expression of 9 proteins in rat testes (GRP78,HSP7C,KNT1,HSP72,ALBU,STIP1,PDIA1,ALDR and GDIR1). PDIA1 and HSP7C were validated by Western blot analysis,and the results were accordance with DIGE analysis. CONCLUSION: Our findings partially supported our early observation regarding seminiferous tubule dysgenesis in rat. In addition,this study revealed technological advancement in reproductive toxicology of SiO₂.

Key words: nano-SiO₂, testis, reproductive toxicology, 2D-DIGE

CLC Number:

R994.3

CUI Shengjin, CHEN Zeyan, CAO Zhaopeng, HUANG Rong, LIU Qing, ZHOU Yiwen. Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 374-378.

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Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats

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Mechanisms for induction of reproductive toxicity by nano-SiO2 particles in SD male rats

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Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats