Carcinogenesis, Teratogenesis & Mutagenesis ›› 2017, Vol. 29 ›› Issue (5): 364-369.doi: 10.3969/j.issn.1004-616x.2017.05.008

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Regulatory effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes

ZHAN Biao1, SUN Beilei2, LUO Sicong1, ZHUANG Hongkai1, HUANG Pengzhou1, ZENG Yi1, CHEN Weifang3, CHEN Weiying2   

  1. 1. 2013 Students of Seven-year Clinical Medicine Program, Shantou University Medical College, Shantou 515041;
    2. Experimental Teaching Center for Preclinical Medicine, Shantou University Medical College, Shantou 515041;
    3. The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2016-09-25 Revised:2017-01-06 Online:2017-09-30 Published:2017-09-30

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Abstract: OBJECTIVE: To investigate the effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes. METHODS: Human epidermal melanocytes were separated,purified and cultured in vitro. These cultures were randomly divided into control and isoimperatorin groups. Cells were cultured with M-254 medium. For the isoimperatorin group,25 μmol/L isoimperatorin in M-254 was added to the cultures. After 48 h of incubation,the morphology of melanocytes was observed under an inverted microscope. Melanin content was assayed by using the NaOH method. The methods of qPCR,Western blot and immunofluorescent were used to analyze the expression of tyrosinase and β-actin. RESULTS: After treatment with 25 μmol/L isoimperatorin for 48 h,dendrites of melanocytes were slightly elongated compared with the untreated cells. In addition,the treatment reduced melanin content (P < 0.01). The results of qPCR,Western blot and immunofluorescence assay consistently indicated that isoimperatorin reduced tyrosinase mRNA and protein,and increased β-actin mRNA and protein. CONCLUSION: Cultured human epidermal melanocytes which were treated with 25 μmol/L isoimperatorin had reduced content of melanin. The observed effect was associated with inhibition of tyrosinase mRNA and protein,and with increased β-actin mRNA and protein.

Key words: melanocyte, isoimperatorin, tyrosinase, β-actin

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