热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究

doi:10.3969/j.issn.1004-616x.2017.05.007

Abstract

Abstract: OBJECTIVE: To investigate expressions of HDJ1 in lung adenocarcinoma tissues and cell lines,and to reveal the role of HDJ1 in invasive ability of lung adenocarcinoma. METHODS: Immunohistochemistry method was used to detect expressions of HDJ1 in lung adenocarcinoma tissues and adjacent normal tissues. Western blot method was applied to determine expressions of HDJ1 in human lung adenocarcinoma cell lines (A549,H1299,H292) and normal lung epithelial cell line (Bease-2B). pMagic 7.1-HDJ1-KD lentivirus was transfected into the A549 cell line to construct a HDJ1 down-expressed cell line,and pMagic 7.1-NC lentivirus was transfected into the same cells as the control. The invasion ability of HDJ1 down-expressed group and control group was analyzed using the Transwell and the Wound Healing assays. RESULTS: The expression levels of HDJ1 in lung adenocarcinoma tissues were significantly higher than those in the adjacent normal tissues. HDJ1 was over-expressed in the A549 cell line. Compared with the control group,the Wound Healing assay results show that,the migration and healing ability of the HDJ1 down-expressed A549 cells were decreased. At 48 h after transfection,both the invasion and migration abilities of the HDJ1 down-expressed A549 cells were lower than that of control cells (P < 0.05). CONCLUSION: Heat shock protein HDJ1 were highly expressed in the lung adenocarcinoma tissues and cell lines. In addition,HDJ1 promoted the invasion and migration ability of lung adenocarcinoma cells.

Key words: heat shock protein, HDJ1, lung adenocarcinoma, invasion, migration

CLC Number:

R730.2

LI Yuxuan, LI Kai, ZHANG Tongtong, GUO Yuanbiao, YIN Jun, BAO Yong. Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 358-363.

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Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells

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