阿魏酸对人脐静脉血管内皮细胞辐射损伤的保护作用及其机制

doi:10.3969/j.issn.1004-616x.2017.05.001

Abstract

Abstract: OBJECTIVE: To study the effect of ferulic acid on damage of human umbilical vein endothelial cells (HUVECs) from their exposure to ionizing radiation METHODS: HUVECs were exposed to ⁶⁰Co gamma ray at the radiation doses of 4-16 Gy. Cells viability as determined by the MTS assay was examined 48 h after irradiation to select the appropriate irradiation dose. Expression levels of Thbd and γH2AX were examined by western blot assay with the total protein of HUVEC extracted at 1,3,6,12,24 and 48 h after irradiation. In addition,HUVEC received 0.001-1 μmol/L ferulic acid at 2 h before irradiation. Cell viability and expression of Thbd and γH2AX protein were determined at 48 hours after irradiation. RESULTS: Compared with the non-irradiated group,cell viability at 48 h after 10 Gy irradiation was decreased by 30% (P < 0.05). Thbd expression was reduced to about 0.6-fold (P < 0.05) at 1 h after irradiation. γH2AX was increased about 5 times (P < 0.05) at 48 h after irradiation. Addition of 0.1 to 1 μmol/L ferulic acid increased the viability of irradiated HUVEC and the protein expression of Thbd at 1 h after irradiation,and decreased the expression of γH2AX at 48 h after irradiation. CONCLUSION: Ferulic acid in the concentration of 0.1-1 μmol/L played a role in radiation protection since it promoted cell vitality and proliferation by increasing the expression of Thbd protein and decreasing the expression of γH2AX protein.

Key words: ferulic acid, anti-radiation, thrombomodulin, γH2AX, vascular endothelial cell

CLC Number:

R994.6

SHAO Shuai, TIAN Mei, LIU Jianxiang, GOU Qiao, QI Xuesong, SU Xu. Protective mechanism of ferulic acid on irradiated human umbilical vein endothelial cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 325-328.

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Protective mechanism of ferulic acid on irradiated human umbilical vein endothelial cells

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