Abstract:

OBJECTIVE: To investigate toxicity of extracts from five kinds of plastic runway surface layer (silicon PU ball surface layer,plastic mat,EPDM plastic track,mixed plastic runway panel,prefabricated plastic track) and to provide a preliminary understanding of its safety to mouse fibroblast L-929. METHODS: Four groups of high density polyethylene negative control,no sample blank control,10% DMSO positive control and 0.5% DMSO extract were used to detect the cytotoxicity of the sample extract on mouse fibroblast L-929 using XTT colorimetry. RESULTS: The relative proliferation rate of the four kinds of samples of silicon PU ball surface layer,plastic mat,EPDM plastic track and mixed plastic runway panel was less than 70%. The relative proliferation rate of the prefabricated plastic runway sample was more than 70%. CONCLUSION: 0.5% DMSO extract showed strong in vitro cytotoxicity of silicon PU ball surface layer,plastic mat,EPDM plastic track,mixed plastic runway panel sample.

Key words: plastic runway, in vitro cytotoxicity, dimethylsulfoxide, XTT staining