LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2017.04.012

Abstract

Abstract:

OBJECTIVE: To investigate the expression of RNA-binding proteins LIN28A,epithelial-mesenchymal transition (EMT) -related proteins E-cadherin and vimentin in invasive breast cancer and to understand their role in development of invasive breast cancer. METHODS: Quantitative real-time PCR (qPCR) and immunohistochemical staining were used to detect expressions of LIN28A,E-cadherin and vimentin mRNAs and proteins in invasive breast cancer tissues and the corresponding adjacent tissues. Relationships between mRNA and protein expressions of LIN28A,E-cadherin and vimentin were analyzed. TUNNEL staining was used to detect apoptosis of invasive breast cancer tissues with high LIN28A expressions. The relationship between LIN28A and EMT-related proteins and survival rate of breast cancer patients were statistically analyzed. RESULTS: qPCR results show that the relative expression level of LIN28A mRNA in breast cancer tissues were higher than adjacent tissues, negatively correlated with E-cadherin mRNA,and positively correlated with vimentin mRNA(P < 0.05). Immunohitochemistry staining results show that in breast cancer tissues,the expression rates of LIN28A,E-cadherin and vimentin protein were 60%,55% and 65%,respectively. The expression of LIN28A protein was negatively correlated with E-cadherin protein,positively correlated with vimentin protein. LIN28A expression was correlated with tumor size,histological grade and lymph node metastasis. TUNNEL assay show that high expression of LIN28A could decrease apoptosis in invasive breast cancer(P < 0.05). The survival rate of patients with positively expressed LIN28A was significantly lower than that of LIN28A negative patients (P < 0.05). CONCLUSION: The RNA-binding protein,LIN28A,may promote the occurrence,development and metastasis of breast cancer via EMT,while high expression of LIN28A may affect prognosis of patients with breast cancer.

Key words: invasive breast cancer, LIN28A, E-cadherin, vimentin, epithelial-mesenchymal transition, apoptosis

CLC Number:

R730.2

LI Youmei, ZHANG Siyi, ZHAO Lianmei, SHAN Baoen, ZHANG Chao. Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 304-308,312.

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Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer

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