维生素E琥珀酸酯诱导人胃癌细胞发生未折叠蛋白反应的研究

doi:10.3969/j.issn.1004-616x.2017.04.003

Abstract

Abstract:

OBJECTIVE: To investigate the effect of vitamin E succinate (VES) on unfolded protein response (UPR) in human gastric cancer SGC-7901 cells. METHODS: SGC-7901 cells were treated with VES at 5,10 and 20 μg/mL and with tunicamycin (TM) at 3 μg/mL for 24 h or at 20 μg/mL for up to 24 h. RNA-dependent protein kinase (PKR)-like ER kinase (PERK) and activating transcription factor 6 (ATF6) were evaluated using Western blotting. X-box-binding protein 1 (XBP1) and activating transcription factor 4 (ATF4) were evaluated using RT-PCR. RESULTS: VES induced the phosphorylation of PERK in a time-dependent manner and reached the top level at 10 μg/mL. In addition, VES induced the generation of p50ATF6 in SGC-7901 cells in a dose-and time-dependent manner. Like the positive control,VES treatment at 20 μg/mL induced the spliced form of XBP1 from 6 h up to 24 h. Meanwhile,ATF4 mRNA was also up-regulated in cells exposed to VES in a time-dependent manner and reached the top level at 18 h. CONCLUSION: UPR pathways may be activated by VES in human gastric cancer SGC-7901 cells.

Key words: unfolded protein response, vitamin E succinate, gastric cancer SGC-7901 cells, endoplasmic reticulum stress

CLC Number:

R730.2

HUANG Xiaoli, WU Kun, ZHAO Shasha. Vitamin E succinate induced unfolded protein response of human gastric cancer SGC-7901 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 256-259,265.

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Vitamin E succinate induced unfolded protein response of human gastric cancer SGC-7901 cells

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