阿特拉津对青春期大鼠黑质区多巴胺能神经元的损伤作用

doi:10.3969/j.issn.1004-616x.2017.04.002

Abstract

Abstract:

OBJECTIVE: To study effects of atrazine (ATR) on neuronal degeneration in adolescent rats. METHODS: 4 weeks old Wistar male rats were randomly subdivided into 4 groups:control and ATR test groups. All rats were treated with ATR:50,100 and 200 mg/kg body weight per day (five times per week) for 45 days by oral gavage. They were sacrificed at 12 h after the last ATR injection. The entire midbrain was collected and stored frozen at -80℃. The presence of tyrosine hydroxylase(TH) was detected by immunohistochemistry SP in dopaminergic neurons. TH expression in mRMA and protein was measured using real-time PCR and Western blot. RESULTS: Immunohistochemical results showed that,compared with the control group,the number of TH positive cells in the 200 mg/kg ATR group was significantly decreased (P < 0.01). In addition,qPCR results show that expression of TH mRNA and Bcl-2 mRNA was significantly decreased in the 100 and 200 mg/kg ATR groups,and expression of p53 mRNA and Caspase-9 mRNA was significantly increased in the 200 mg/kg ATR group,the difference was statistically significant (P < 0.05). Western blot results show that expression of TH and Bcl-2 protein was significantly decreased in the 100 and 200 mg/kg ATR groups compared with the control group. Expression of p53 protein in the ATR group was significantly increased at 100 and 200 mg/kg,and Caspase-9 protein in he 200 mg/kg ATR group was significantly increased,the difference was statistically significant (P < 0.05). CONCLUSION: Atrazine caused damage to the dopaminergic neurons in the substantia nigra of rats and activated the apoptotic pathway which led to the dopaminergic neuronal death.

Key words: atrazine, dopaminergic neurons, immunohistochemistry, tyrosine hydroxylase

CLC Number:

R741

MA Kun, WU Haoyu, LI Peng, WANG Li, LI Baixiang. Induction of dopaminergic neuron degeneration by atrazine in substantia nigra in adolescent rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 251-255.

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Induction of dopaminergic neuron degeneration by atrazine in substantia nigra in adolescent rats

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