铁过载对性成熟期雄性大鼠生育能力的影响

doi:10.3969/j.issn.1004-616x.2017.02.012

Abstract

Abstract:

OBJECTIVE: The aim of the study was to observe the effect of iron overload on fertility of sexually mature male rats. METHODS: According to bodyweights,40 healthy male Wistar rats were randomly divided into four groups:control,iron deficiency,low-dose iron overload and high-dose iron overload groups. All rats were injected intraperitoneally every other day. The content of the Fe2+ in these four groups were 0.9,0.3,9 and 18 mg,respectively. After 6 weeks,blood samples from these rats were collected to determine serum iron levels. Testes and epididymides were removed and weighed. Sperm numbers were counted. Sperm mobility and teratosperm rates were calculated. Iron concentration,catalase (CAT),total superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px) and malonaldehyde (MDA) levels in the testes were determined. RESULTS: Compared with the control group,the serum iron concentration of the iron deficiency group was significantly lower (P < 0.01). However,there was no significant difference in the iron concentrations of the testes (P > 0.05). The serum and testes iron concentrations in the low- and high-doses iron overload groups were both significantly higher than that in the control group (all P < 0.01). There was no significant difference in the sperm count,the sperm motility rate and the teratosperm rate between the iron deficiency group and the control group (all P > 0.05). Compared with the control group,the sperm motility rate in the low-dose iron overload group was significantly decreased (P < 0.01);the sperm count and the sperm motility rate in the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the teratosperm rate was significantly higher than that of control group and low-dose iron overload group (all P < 0.05). The rats in the iron deficiency group had significantly lower GSH-Px levels in the testis comparing with the control group. Moreover,compared with the control group,the GSH-Px levels in the testis of the low-dose iron overload group were significantly lower,but the MDA level was significantly higher (all P < 0.05). The CAT and GSH-Px levels in the testis of the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the MDA level was significantly higher than that of control group and low-dose iron overload group (all P < 0.05). CONCLUSION: Iron overload can affect the fertility of sexually mature male rats,including reduced sperm count,decreased sperm motility rate and increased teratosperm rate. These adverse effects may be related to the increased oxidative stress level caused by iron overload.

Key words: iron overload, sperm count, sperm motility rate, teratosperm rate, oxidative stress

CLC Number:

R321.1

ZHANG Huaqi, MA Aiguo, SUN Yongye, ZHONG Feng, CAI Jing, HE Limin. Effect of iron overload on fertility of sexually mature male rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 134-138,142.

References

[1] ZHANG A S,ENNS C A. Iron homeostasis:Recently identified proteins provide insight into novel control mechanisms [J]. J Biol Chem,2009,284(2):711-715．

[2] KARTIKASARI A E,GCORGION N A,VISSEREN F L,et al. Endothelial activation and induction of monocyte adhesion by nontransferrin-bound iron present in human sera[J]. FASEB J,2006,20(2):353-355.

[3] 赵长峰,杨洪旗,姜会敏,等．铁缺乏对孕鼠及其胎鼠体内元素分布的影响[J]．卫生研究,2001,30(5):277-279．

[4] MEANS R J. Advances in the anemia of chronic disease[J]. Int J Hematol,1999,70:7-12.

[5] ANDREWS N C. Medical progress:disorders of iron metabolism[J]. N Engl J Med,1999,341:1986-1995．

[6] NICOLAS G,VIAAE L,LOU D Q,et al. Constitutive hepcidin expression prevents iron overload in a mouse model of hemochromatosis[J]. Nat Genet,2003,34:97-101.

[7] 张梦雪,马爱国,胡迎芬,等．铁摄入过量对大鼠胚胎发育营养的实验研究[J] ．中国食物与营养,2014,20(2):65-69．

[8] 王心如. 毒理学基础[M]. 北京:人民卫生出版社,2003:119．

[9] HAMETNER S,WIMMER I,HAIDER L,et al. Iron and oxidative damage in the multiple sclerosis brain[J]. J Neurol Sci,2013,333(11):386-387.

[10] 李新华,张欣欣. 铁负荷过多综合征的研究进展—2007 年美国肝病学会 (AASLD) 年会专题报道[J]. 肝脏,2008,13(2):148-150.

[11] MIRA MALKOV,YONIT FISHER,JEREMY DON. Developmental Schedule of the postnatal rat testis determined by flow cytometry[J]. Biol Reprod,1998,59:84-92.

[12] 陈绍坤,余红,李洁,等. 微量元素与男性不育症患者精子质量的关系分析[J]. 现代医药卫生,2012,28(6):849-850.

[13] 何丽敏,孙永叶,蔡静,等. 铁过量对大鼠淋巴细胞DNA损伤的影响[J]. 癌变·畸变·突变,2015,27(1):59-63.

[14] 王亚锦,胡迎芬,王洋,等. 铁过载对大鼠骨髓及肝脾组织的影响[J]. 现代生物医学进展,2015,15(19):3641-3644.

[15] 徐谊朝,朱伟杰,李菁,等. 有生育需求中老年男性精子参数的评价[J]. 生殖与避孕,2014,34(007):599-602.

[16] NASRI S,REZAI-ZARCHI S,KERISHCHI P,et al. The Effect of iron oxide nanoparticles on sperm numbers and mobility in male mice[J]. Zahedan J Res Med Sci,2015:29-31.

[17] JALILI C,SALAHSHOOR M R,NASERI A. Protective effect of Urtica dioica L against nicotine-induced damage on sperm parameters,testosterone and testis tissue in mice[J]. Iran J Reprod Med,2014,12(6):401-408.

[18] WANG J,PANTOPOULOS K. Regulation of cellular iron metabolism[J]. Biochem J,2011,434(1):365-381.

[19] 陈玉明,陈杰,王日文,等. Fe²⁺对雄性泥鳅生殖毒性的研究[J]. 湖北农业科学,2011,50(7):1444-1447.

[20] FINAZZI D,AROSIO P. Biology of ferritin in mammals:an update on iron storage,oxidative damage and neurodegeneration [J]. Arch Toxicol,2014,88(10):1787-1802.

[21] 李云春,马爱国,汪求真,等. 叶黄素对铁过载大鼠氧化应激水平影响的实验研究[J]. 中国食物与营养,2014,20(3):58-61.

[22] MOJICA-VILLEGAS M A,IZQUIERDO-VEGA J A,CHAMORRO-CEVALLOS G,et al. Protective effect of resveratrol on biomarkers of oxidative stress induced by iron/ascorbate in mouse spermatozoa[J]. Nutrients,2014,6(2):489-503.

[23] WELLEJUS A,POULSEN H E,LOFT S. Iron-induced oxidative DNA damage in rat sperm cells in vivo and in vitro[J]. Free Radic Res,2000,32(1):75-83.

[24] MARZEC-WRÓBLEWSKA U,KAMI?SKI P,?AKOTA P,et al. Zinc and iron concentration and SOD activity in human semen and seminal plasma[J]. Biol Trace Elem Res,2011,143(1):167-177.

[25] MATÉS J M,PÉREZ-GÓMEZ C,NUNEZ D C. Antioxidant enzymes and human diseases[J]. Clin Biochemi,1999,32(8):595-603.

Effect of iron overload on fertility of sexually mature male rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	AI Duo, XU Anqi, KONG Deqin, LIU Ying, YU Weihua, WANG Zhao, PENG Jie, LIU Rui, ZHANG Xiaodi, HAI Chunxu, LI Wenli, WANG Xin, LIU Jiangzheng. Protective effects of catalytic antioxidant AEOL-10150 against nitrogen mustard-induced acute liver injury in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 40-46,61.
[2]	LI Hongwei, KONG Deqin, YU Weihua, WU Hao, WANG Zhao, LIU Rui, HAI Chunxu, WANG Xin, LIU Jiangzheng, LI Wenli. Protective effect of oleanolic acid co-treatment on alcohol-induced oxidative damage in gastric mucosa of rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 365-369.
[3]	JIANG Shan, DU Feng, KANG Bingwen, YIN Caocao, ZHOU Jian, SHI Ying, WANG Yue, ZHOU Gengyao, QIN Xujun. Attenuation of D-galactose-induced senescence of human umbilical vein endothelial cells by isorhamnetin [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 95-100.
[4]	ZHAO Junwei, HUA Chenfeng, SHANG Pingping, XIE Fuwei, LI Xiang. Induction of oxidative stress by arsenious acid As(Ⅲ) in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 28-31.
[5]	SUN Jiaojiao, CHE Bizhong, LUO Qiulin, ZHAI Bingzhong, XIN Lili. 1,25-(OH)₂D₃ attenuates PM_2.5-induced oxidative stress in HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 92-97.
[6]	JIN Lei, ZHOU Jiayou, HAN Jiacheng, GUAN Haojun, WANG Shuai, PENG Jie, WANG Xin, HAI Chunxu, LI Wenli. Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 21-28.
[7]	ZHANG Xuguang, DU Ruihu, CHEN Yanping, ZHAO Yan. Effect from activation of acid sphingomyelinase and ceramide on vitamin E succinate-induced apoptosis in human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 379-384.
[8]	LIAO Nai, LONG Zi, HAI Chunxu, WANG Xin. 2,3,7,8-tetrachlorodibenzo-p-dioxin alone or in combination with high fat diet on glucose and lipid metabolism in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 111-118.
[9]	GUO Haidong, YANG Runfang, LIU Xinyu, LIU Junjun, HE Jin, GAN Ziqiong, LI Hongxia. Oxidative stress response in rats after exposure to fire-drill prop smoke [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 41-44,48.
[10]	WEN Hairuo, GUO Yajuan, HUANG Zhiying, WANG Xue, DAN Mo. Induction of cytotoxicity and DNA damage by iron oxide nanoparticles with different surface modifications in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 413-421.
[11]	CAO Yue, SUN Mengyun, CAI Jingming, ZHANG Lijia, ZHAO Yan. Involvement of miR-34a and its downstream genes plus iron overload in rats with nonalcoholic fatty liver disease [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 378-383.
[12]	ZHOU Xiaoqing, WEN Kexin, YIN Hongping, YANG Jinru, ZHU Yongfei. Effects of intrauterine flutamide exposure on uterus and ovary development and on oxidative stress among female offspring [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 384-388,394.
[13]	WANG Yuan, DENG Xiaofei. Effect of DNA glycosylase OGG1 against Helicobacter pylori-induced DNA damage in gastric epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 92-97,102.
[14]	ZHOU Qingbiao, LIU Ying, KONG Deqin, ZHANG Jiaxin, YU Weihua, BAI Hua, HAI Chunxu. Protective effect of selenium in chronic cadmium exposure-induced kidney injury in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 1-7.
[15]	WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 379-382.