Carcinogenesis, Teratogenesis & Mutagenesis ›› 2017, Vol. 29 ›› Issue (2): 125-128,133.doi: 10.3969/j.issn.1004-616x.2017.02.010

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Radioprotection effects from different extracts of Carthamus tinctorius

FAN Li, LIU Jianxiang, FENG Jiangbin, JIANG Xiaoyan, GAO Gang, TIAN Mei   

  1. National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2016-12-06 Revised:2017-01-22 Online:2017-03-31 Published:2017-03-31

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Abstract:

OBJECTIVE: To develop new,safe and effective radioprotectors,the protective effects from different water extracts of Carthamus tinctorius were investigated. METHODS: Safflower water extract was prepared with distilled water at 60 ℃ and isolated by HP-20 macroporous resin. Water elution fraction,30%,50%,70% and 95% ethanol elution fractions were separated for the study. The free radical scavenging ability of the different extraction fractions were evaluated by electron spin resonance (ESR) spectra. CCK-8 assay was performed to evaluate the survival rate of human liver L02 cells in vitro. Flow cytometry was applied to study apoptosis,cell cycle and the ratio of Bcl-2/Bax of human lymphocyte AHH-1 after 60Co ay irradiation. RESULTS: The ESR results showed that all the different effective parts of safflower water extract had the ability to scavenge free radicals,with IC50 at 1.7-79.7 mg/mL. The survival rates from the 30% and 50% ethanol elution were 128.3% and 130.9% by the CCK-8 assay. The survival rates were significantly higher than the irradiated group and suggested that the two parts had equal radioprotection effect to the water extract of safflower. The decreased apoptosis rate of AHH-1 cells:the 30% fraction (15.3%±1.6%) and the 50% fraction(15.2%±1.9%) were significantly lower than that of the irradiation group (24.7%±4.4%) (P < 0.01). The results of AHH-1 cell cycle analysis showed that the 30% and 50% ethanol elution fractions had significant improvements in the G2/M phase block that was induced by irradiation (P < 0.01). At the same time,the two fractions increased the Bcl-2/Bax ratio (P < 0.05) and inhibited the cell apoptosis. CONCLUSION: Our data show that the 30% and 50% ethanol elution fractions had good radioprotective effects for L02 and AHH-1 cells which were irradiated with 60Co γrays. The mechanism for the protection may be related to the elimination of free radicals,the improvement of G2/M cell cycle block,and the regulation Bcl-2/Bax ratio in order to inhibit cell apoptosis. The two fractions should be further studied as potentially safe and effective radioprotectors.

Key words: Carthamus tinctorius L., effective fraction, electron spin resonance spectra, CCK-8, mechanism of radioprotection

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