酵母SUP4-o突变检测系统及铬遗传毒性分析

doi:10.3969/j.issn.1004-616x.2017.01.013

Abstract

Abstract:

OBJECTIVE: To evaluate genotoxicities of chromium (tri- and hexavalent states) using a new yeast mutation SUP4-o assay system. METHODS: YCpMP2 plasmid was transferred into MKP-o (SJR576) to obtain SJR576-p strain by electroporation. After treatment of the yeast cell SJR576-p with the same concentrations of Cr³⁺ and Cr⁶⁺ for 24 h,the cells were coated on canavanine resistance screening plates in uracil synthetic medium (SC-Ura),and the number of clones were subsequently counted. RESULTS: Compared with the untreated controls,the mutation rates of the treated groups with potassium dichromate and chromium trichloride increased five times. The values were about 23 and 26 parts per million,respectively. While there was no significant difference between the groups treated with Cr³⁺ and Cr⁶⁺. CONCLUSION: Our data suggest that Cr³⁺ can cause DNA damage and genotoxicity. Additionally,the yeast SUP4-o mutation assay system is useful for detecting genotoxicity.

Key words: yeast, genotoxicity, mutations, Cr³⁺, Cr⁶⁺

CLC Number:

Q319+.33

MENG Xiaoqing, FANG Zhijia, WEI Hongyan, YANG Zhanju, HUANG Zhiwei. Genotoxicity investigation of tri- and hexavalent chromium using a yeast SUP4-o mutation assay[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 65-69.

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Genotoxicity investigation of tri- and hexavalent chromium using a yeast SUP4-o mutation assay

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