儿茶素对顺铂所致人胚肾细胞氧化损伤的影响

doi:10.3969/j.issn.1004-616x.2017.01.010

Abstract

Abstract:

OBJECTIVE: To investigate the mechanism and effect of catechin on cisplatin (DDP)-induced oxidative damage in HEK293 cells. METHODS: Survival HEK293 cells in vitro after their exposure to catechin or DDP was determined. In addition,the effect of catechin pretreatment on DDP-induced cell growth inhibition was determined by the MTT method. Superoxide dismutase (SOD) activity was examined by the xanthine oxidase method. Malondialdehyde (MDA) content was measured by the thiobarbituric acid method. Glutathione (GSH) content was determined by the nitrobenzoic acid method. RESULTS: Cell survival rate decreased gradually as the DDP concentrations increased. Pretreatment with catechin caused increased cell survival initially but decreased gradually afterward. The initial increase was not significant (P > 0.05). However,catechin treatment significantly increased the intracellular MDA content,but decreased the SOD activity and GSH level compared with that in the DDP control group (P < 0.05). CONCLUSION: Although catechin pretreatment did not improve the survival rate of DDP-treated HEK293 cells,it alleviated the DDP-induced oxidative damage.

Key words: catechin, cisplatin, nephrotoxicity, antioxidant

CLC Number:

R994.1

WANG Hai, LIAN Yanna, GAO Liping, QU Lijie, ZHANG Dongping. Effect of catechin on cisplatin-induced oxidative damage in human embryonic kidney cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 51-54.

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Effect of catechin on cisplatin-induced oxidative damage in human embryonic kidney cells

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