RNAi沉默p53基因对HeLa细胞核转录因子3表达及其对细胞增殖和侵袭能力的影响

doi:10.3969/j.issn.1004-616x.2017.01.009

Abstract

Abstract:

OBJECTIVE: To observe the effect of RNAi silenced p53 gene on the expression of nuclear transcription factor STAT3 in cervical cancer HeLa cells,and on their proliferation and invasion. METHODS: 3 kinds of short hairpin RNA (shRNA) which target the p53 gene were constructed and were used for liposome-transfection into HeLa cells. Then,expression of p53 were detected by PCR and Western blot. After silencing the p53 gene,expression of STAT3 was detected by PCR and Western blot. Proliferation and invasion of HeLa cells were detected by the MTT and Transwell assays. RESULTS: The transfection efficiency of p53-shRNA3 group was 85.7%. After the transfection,the expression of p53 mRNA and protein were 0.23±0.05 and 0.41±0.11,respectively,significantly lower than the non-transfection,empty plasmid,p53-shRNA1 and p53-shRNA2 groups (P < 0.05). In addition,expression of STAT3 mRNA and protein of the p53-shRNA3 group were 1.09±0.21 and 1.46±0.25,respectively,significantly higher than non-transfection group and empty plasmid group (P < 0.05). MTT results showed that at 24,36,48 h and 72 h,cell proliferation ability of the p53-shRNA3 group was significantly higher than the non-transfected group and empty plasmid group (P < 0.05). Transwell results showed that the number of cells in the p53-shRNA3 group (0.05±59.36) was significantly higher than the non-transfection group (15.73±1.29) and the empty vector group (16.01±1.34) (P < 0.05). CONCLUSION: Silencing the p53 gene can significantly increase the expression of STAT3,and promote the proliferation and invasive ability of HeLa cells.

Key words: RNAi silencing, p53, HeLa cell, signal transducer and activator of transcription 3, proliferation, invasion

CLC Number:

R730.231

LI Huiying, LI Yang. Effect of RNAi silenced p53 gene on expression of nuclear transcription factor 3 in cervical cancer HeLa cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 46-50.

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Effect of RNAi silenced p53 gene on expression of nuclear transcription factor 3 in cervical cancer HeLa cells

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