肉苁蓉苯乙醇总苷及其单体对肝星状细胞增殖和凋亡的影响

doi:10.3969/j.issn.1004-616x.2017.01.003

Abstract

Abstract:

OBJECTIVE: To explore the effects of cistanche phenylethanoid glycosides (CPhGs),acteoside and echinacoside on anti-proliferation and induction of apoptosis in hepatic stellate cells (HSC-T6) in vitro. METHODS: HSC-T6 cells were treated with different concentrations of CPhGs (25,50,100 μg/mL),acteoside (1.5,3,6.0 μg/mL) or echinacoside (125,250,500 μg/mL) for 48 h. The MTT methods was used to measure the anti-proliferation effect and half the inhibition rate (IC₅₀) of HSC-T6. Proportions of apoptosis were assayed by flow cytometry. Western blot was used to detect the effects of the expression of Bcl-2 and Bax. RESULTS: With increasing concentrations of the three chemicals,the rate of inhibition of proliferation increased gradually. IC₅₀ were 119.1,7.0 and 520.3 μg/mL for the three chemicals,respectively. The proportions of apoptosis for the three chemicals were significantly different from that of the untreated controls (P < 0.01). The highest apoptosis rates for CPhGs (at 100 μg/mL),acteoside (6.0 μg/mL) and echinacoside (500 μg/mL) were 24.40%±3.16%,34.73%±1.16% and 21.13%±0.98%,respectively. Except for the 1.5 μg/mL acteoside group,expression of Bcl-2/Bax was significantly decreased (P < 0.05) compared to the normal control group. CONCLUSION: CPhGs,echinacoside and acteoside caused proliferation inhibition and apoptosis. The sequence of the hepato-protective effects of the three chemicals was shown to be acteoside > CPhGs > echinacoside.

Key words: cistanche phenylethanoid glycosides, acteoside, echinacoside, hepatic stellate cells, apoptosis

CLC Number:

YOU Shuping, ZHAO Jun, MA Long, ZHANG Shilei, LIU Tao. Effects of cistanche phenylethanoid glycosides, acteoside and echinacoside on inhibition of cell proliferation and inducation of apoptosis in rat hepatic stellate cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 13-17,22.

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Effects of cistanche phenylethanoid glycosides, acteoside and echinacoside on inhibition of cell proliferation and inducation of apoptosis in rat hepatic stellate cells

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