干酪乳杆菌对二甲基苯蒽诱发大鼠乳腺肿瘤的抑制作用及其免疫学机制

doi:10.3969/j.issn.1004-616x.2017.01.002

Abstract

Abstract:

OBJECTIVE: To investigate the effect of Lactobacillus casei on 7,12-dimethylbenz(a)anthracene (DMBA) induction of breast cancer in rats. METHODS: Female Spraque-Dawley rats were randomly divided into control group (C),model group (M),low dose group of Lactobacillus casei (L1) and high dose group of Lactobacillus casei (L2). The rats were subcutaneously injected with 100 mg/kg DMBA to establish breast cancer rat model. 4 and 8 mL/kg Lactobacillus casei were administered to L1 and L2 by gavage,respectively,1 time/day,for 16 weeks in total. The latency of breast cancer in rats and Lactobacillus casei's tumor suppression rate were determined. The percentages of T lymphocytes and natural killer cells were quantified in peripheral blood of rat by flow cytometry. In addition,thymus index,spleen index and the level of interleukin 4(IL-4),IL-6,IL-10,IL-12,interferon γ (IFN-γ),and tumor necrosis factor α (TNF-α) in serum were evaluated. RESULTS: No tumor was detected in group C,whereas tumors were formed in the remaining three groups. Compared with the model group,the L2 group had extended latency of tumors,reduced tumor incidence and tumor weights (P < 0.05). In addition,the tumor inhibition rate in the same group reached 41.2%,the thymus index was increased significantly (P < 0.05),the percentage of TCRαβ⁺ CD161a⁺ NK cells and the CD3⁺CD8⁺ T cells were both increased significantly (P < 0.05),while the proportion of CD3⁺Foxp3⁺ T cells was decreased significantly (P < 0.05). ELISA results showed that,compared with the control group,the concentration of IL-4 was decreased in the model group while the concentrations of IL-6,IL-12 and TNF-α were increased (P < 0.05). In the L2 group,the concentration of IL-4 and IL-10 were significantly higher than those in the model group while the concentrations of IL-6 and IL-12 were decreased respectively (P < 0.05). In the L1 and L2 groups,TNF-α concentrations were decreased significantly compared with the model group (P < 0.05). CONCLUSION: Lactobacillus casei supplementation can inhibit the induction of tumors by DMBA in rats. A possible mechanism may involve immune-regulatory effects on the breast cancer rat model via improving the levels of inflammation-associated cytokines and changing the distribution of CD3⁺CD4⁺,CD3⁺CD8⁺ T cells,NK cells and regulatory T cells.

Key words: breast carcinoma, Lactobacillus casei, immune regulation, anti-tumor

CLC Number:

R392.1

LIANG Zhengyan, LIU Ying, XUE Meilan, LIU Jia, LIANG Hui. Inhibitory effect and immunological mechanism of Lactobacillus casei on induction of breast cancer by 7, 12-dimethylbenz (a)anthracene in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 7-12.

References

[1] ANDERSON B O,JAKESZ R. Breast cancer issues in developing countries:an overview of the breast health global initiative[J]. World J Surg,2008,32(12):2578-2585.

[2] SHARIFIAN A,POURHOSEINGHOLI M A,EMADEDIN M,et al. Burden of breast cancer in Iranian women is increasing[J]. Asian Pac J Cancer Prev,2015,16(12):5049-5052.

[3] BEDOGNETTI D,MACCALLI C,BADER S B,et al. Checkpoint inhibitors and their application in breast cancer[J]. Breast Care,2016,11(2):108-115.

[4] DOMSCHKE C,SCHNEEWEISS A,STEFANOVIC S,et al. Cellular immune responses and immune escape mechanisms in breast cancer:determinants of immunotherapy[J]. Breast Care, 2016,11(2):102-107.

[5] 匡栩源,庄权,吴金泽,等.益生菌在肿瘤防治中的机制和应用[J].中国微生态学杂志,2011,23(3):274-276.

[6] LóPEZ P,GONZáLEZ-RODRíGUEZ I,SáNCHEZ B,et al. Interaction of bifidobacterium bifidum LMG13195 with HT29 cells influences regulatory-T-cell-associated chemokine receptor expression[J]. Appl Environ Microbiol,2012,78(8):2850-2857.

[7] SIVAN A,CORRALES L,HUBERT N,et al. Commensal bifidobacterium promotes antitumor immunity and facilitates anti-PD-L1 efficacy[J]. Science,2015,350(6264):1084-1089.

[8] 伊淑帅,王开,齐宇,等.干酪乳杆菌与氟苯尼考对肉雏鸡生长性能及免疫力影响的比较研究[J].中国兽药杂志,2015,49(9):31-36.

[9] HAN D J,KIM J B,PARK S Y,et al. Growth inhibition of hepatocellular carcinoma huh7 cells by Lactobacillus casei extract[J]. Yonsei Med J,2013,54(5):1186-1193.

[10] DALLAL M M S,MOJARRAD M,BAGHBANI F,et al. Effect of probiotic Lactobacillus acidophilus and Lactobacillus casei on colorectal tumor cells activity (CaCo-2)[J]. Arch Iran Med, 2015,18(3):167-172.

[11] 董春景,梁惠,贺娟,等.海藻萜类化合物对二甲基苯蒽诱导的大鼠乳腺癌的抑制作用[J].营养学报,2007, 29(2):197-199.

[12] TAVASSOLI F A,DEVILEE P. WHO:乳腺及女性生殖器官肿瘤病理学和遗传学[M].程虹,戴林,郭双平,等译.北京:人民卫生出版社,2006:1-118.

[13] 托娅,杜瑞亭,张和平.益生菌Lb.casei Zhang对H22荷瘤小鼠的抗肿瘤作用及机制[J].肿瘤防治研究,2010, 37(4):463-465.

[14] 吴英韬,解傲,袁杰利.多联益生菌对5-氟尿嘧啶诱发大鼠肠道菌群紊乱及肠黏膜损伤的保护作用[J].中国微生态学杂志,2016,28(4):378-382.

[15] 胡帅尔,陈壁锋,李文立,等.益生菌对小鼠特异性及非特异性免疫功能的影响[J].公共卫生与预防医学,2010, 21(4):11-14.

[16] HORINAKA M,YOSHIDA T,KISHI A,et al. Lactobacillus strains induce TRAIL production and facilitate natural killer activity against cancer cells[J]. FEBS Lett,2010,584(3):577-582.

[17] 周金黄,李晓玉,荣康泰.免疫药理学进展基础与临床[M].北京:科学出版社,1993:353-354.

[18] 娄鸿飞,房居高,李平栋,等.CD4⁺CD25⁺Foxp3⁺调节性T细胞在鼻腔鼻窦鳞状细胞癌中的表达和免疫抑制功能研究[J]. 中国耳鼻咽喉头颈外科,2015,22(6):299-303.

[19] 高祥,郭涛,黄婧,等.体外扩增的Treg细胞抑制NK细胞介导的抗肿瘤免疫[J].免疫学杂志,2015,31(4):308-312.

[20] OGITA T,BERGAMO P,MAURANO F,et al. Modulatory activity of Lactobacillus rhamnosus OLL2838 in a mouse model of intestinal immunopathology[J]. Immunobiology,2015, 220(6):701-710.

[21] MORTAZ E,ADCOCK I M,RICEIARDDO F L,et al. Anti-inflammatory effect of Lactobacillus rahmnosus and Bifidobacterium breve on cigarette smoke activated human macrophages[J]. PLoS One,2015,10(8):1-16.

[22] HORMANNSPERGER G,VON SCHILLDE M A,HALLER D. Lactocepin as a protective microbial structure in the context of IBD[J]. Gut Microbes,2013,4(2):152-157.

[23] HALLER D,BENGMARK S,ENCK P,et al. Guidance for substantiating the evidence for beneficial effects of probiotics:probiotics in chronic inflammatory bowel disease and the functional disorder irritable bowel syndrome[J]. J Nutr,2010, 140(3):690S-697.

[24] ROCHE H,VIENS P,BIRON P,et al. High-dose chemotherapy for breast cancer:the french PEGASE experience[J]. Cancer Control,2003,10(1):42-47.

Inhibitory effect and immunological mechanism of Lactobacillus casei on induction of breast cancer by 7, 12-dimethylbenz (a)anthracene in rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHU Xinping, JIA Di, LIN Shaohui, LIU Chen, LI Jingyi, ZHAO Weiming. Inhibitory effects of a novel polyoxometalate drug {BiW₈O₃₀}on liver cancer SK-HEP-1 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 47-52,66.
[2]	LIU Ning, LIANG Lanlan, LI Shufang, SHEN Xiangchun, LIANG Bing. Anti-tumor effects of oxymatrine on mouse sarcoma and live cancers in vivo and in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 426-429,434.
[3]	YANG Meiling, LI Xin, HUANG Junming, ZHANG Zihong, YI Zimei, GU Meiying, HUANG Jiankang, HE Yun. Enhancement of anti-tumor effect of cisplatin by chrysin in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 37-41,59.
[4]	FU Ying, ZHAO Chen, CHANG Zhenzhan, LIN Huaying, SUN Zhenxiao. Inhibition effects of Arca subcrenata Lischke anticancer protein on human breast carcinoma MCF-7 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 415-420.
[5]	KANG Xinmei，WANG Hui，WANG Li，TANG Dabei，ZHANG Qingyuan. Effect of daidzein on the growth and angiogenesis of breast carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(1): 16-20.
[6]	SHEN Yan-mei，SHAN Bao-en，LIU Li-hua，ZHANG Chao，ZHOU Kai-xuan，JIAO Yu-qian，JIAO Wen-jing. The anti-tumor effects of HSP70 peptide complex [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(4): 262-266.
[7]	YAN Xi，ZHAO Lian-mei，SUN Jia-wei，SHAN Bao-en. In vitro anti-tumor activity of Forsythia suspensa extract [J]. , 2012, 24(1): 20-24.
[8]	ZHAN Li-juan1; WANG Ai-ping1;HOU Xiao-ming1;LIN Xiao-qi1;LI Yan2. The physical tolerance, immune-regulating and antineoplastic effects of NJY lyophilized powder [J]. , 2010, 22(3): 221-225.
[9]	ZHAO Lian-mei; HAN Li-na;SHAN Bao-en;PAN Xiao-ming;LIU Li-hua; LI Xiao-guang. Anti-tumor activity of cochinchina momordica seed extract [J]. , 2010, 22(1): 19-0023.
[10]	LIANG Yun_xia,CHEN Mao_huai,HUANG Bo,WU Xian_ying. The Significance of Extracellular Matrix Metalloproteinase Inducer and Matrix Metalloproteinase_9 Expression in Breast Carcinoma [J]. , 2007, 19(4): 298-301.
[11]	LIN Fei, LU Qi-xiang, LU Lian, LIANG Yi. Inhibitory Effect of Extracts of Digestive Gland on Proliferation of Tumor Cells [J]. , 2007, 19(2): 116-118.
[12]	ZHOU Ze-wei, SHEN Xiu, WU Xiao-xia, LIU Pei-xun, XU Wen-qing, LI Rui. Experimental Research on Anti-tumor Activities of Natural Osthole [J]. , 2007, 19(2): 119-121.
[13]	ZHU Jing-tong，BAI Yu ,SI Wen-xiu ，LIU Hong-xi，REN Qu-hui. Study About Anti-tumor Effect of Pulsatilla Chinensis(Bunge)Regel Extracts in Vitro [J]. , 2007, 19(1): 67-069.
[14]	ZHANG Jing1; SHAN Bao-en1; LIU Gang-san2; ZHAO Xue-tao1; CHEN Shu-hong2. Anti-tumor Activity of Extract from Periploca Sepium Bge [J]. , 2006, 18(2): 108-111.
[15]	WAN Rong; XIAO Zhi-yun;WANG Hai-yan;GAO Mei-qin. Clinicopathological Significance of Cystatin M Gene Expression in Human Breast Cancer [J]. , 2006, 18(1): 54-057.